Non-crop plants of the Poaceae family mainly perennial weed species such as Bermuda grass (Cynodon dactylon), are host of several viruses and can act as reservoir hosts for them. Therefore, the study of viruses in weed is a critical step in the control of viruses. Cynodon chlorotic streak virus (CCSV) has been reported to infect Bermuda grass and corn. The virus belongs to the familyRhabdoviridaeand the genuucleorhabdoviruand is transmitted byToya propinquain a circulative-propagative manner. To detect CCSV, Bermuda grass samples with chlorotic streaks symptoms on leaves were collected from Isfahan, Chaharmahal-va-Bakhtiari, and Fars provinces. In the RT-PCR technique, using the universal primer pair of rhabdoviruses (RhabF / RhabR), the expected size of the PCR product (970 bp) was amplified in the samples. In the BLAST search, the sequence of the amplified segment showed high degree of homology to an isolate of CCSV reported from South Africa available at GenBank with the accession number of EU650683.1. To determine the nucleotide sequence of the Iranian isolate of CCSV (CCSV-IR), total RNA was extracted, and its quantity, purity, and quality was evaluated using spectrophotometry and electrophoresis. Total RNA was sent for RNA sequencing. RNA-Seq analysis showed that CCSV-IR has six genes on its genome same other nucleorhabdoviruses as 3'-N-P-Gene 3-M-G-L-5'. Multiple alignment based on the nucleotide and amino acid sequences of CCSV-IR genes and those of other rhabdoviruses available at GenBank using DNAMAN 4.0 and CLC Viewer softwares was done. Multiple alignment showed CCSV-IR has the highest similarity to maize Iranian mosaic virus (MIMV), Morogoro maize-associated virus (MMaV), taro vein chlorosis virus (TaVCV), and maize mosaic virus (MMV) as 75 %, 63.9 %, 62.4 %, and 62.2 %, respectively. Phylogenetic analysis based on the amino acid sequence of the L gene of CCSV-IR and those of other rhabdoviruses with Neighbor-joining method revealed CCSV-IR is closer to MIMV than other nucleorhabdoviruses. For determination of genetic variation of Iranian isolates of CCSV, the 970 bp segment of five isolates, including CCSV-IR and CCSV-Esf from Isfahan, CCSV-Fra from Fraidan CCSV- Sha from Shahrekord, and CCSV-Shi from Shiraz were sequenced. Multiple alignment of the amino acid sequence of 970 bp of CCSV Iranian isolates using DNAMAN 4.0 software with the Neighbor-joining method revealed high identity amongst Iranian isolates ranging from 95.3 %-99.2 %. For determining the host range of CCSV, in transmission test includingAvena sativa, Triticum sativum, Oryza sativa, Hordeum vulgare, Zea mays, Sorghum bicolor, Secale montanum, Panicum miliaceum, Bromus tectorum, Secale sp. Hordum murinumandCynodon dactylon,Toya propinquatransmitted CCSV from infected Bermuda grass toHordeum vulgare, Zea mays, Avena sativa,andCynodon dactylon. Our results showed that the closest virus to CCSV-IR is MIMV. Due to transmission of CCSV to essential plants such as barley and corn and abundance of its vectorT. propinquain nature, it seems that CCSV can infect cereals in nature and damage them. Therefore, detection of CCSV in graminaceous plants in nature and estimation of its risk in the future researches should be considered. Key words: Nucleorhbdovirus, cynodon chlorotic streak virus, Host range, genome sequence.