Hyaluronic acid is a sulfate-free, nonlinear glycosamino glycan with repetitive disaccharide units derived from two major animal sources, such as the rooster comb and bacteria. Hyaluronic acid is found in different tissues such as umbilical cord, the synovial fluid between joints, skin, and the vitreous body of the eye. Due to polyanionic properties of hyaluronic acid, it can interact with a natural polycationic polymer such as chitosan to produce layer by layer structures like films or polyelectrolyte fibers with suitable mechanical properties. In this study, hyaluronic acid was first extracted from rooster comb waste by solvent method. The properties of the extracted hyaluronic acid, such as its molecular weight and residual solvent, along with the FTIR and cell toxicity studies showed that the extracted hyaluronic acid with molecular weight of 253KDa has similar structure to that of commercial hyaluronic acid samples and can be used for medical purposes due to its nontoxicity. The next step was to measure the zeta potential for Hyaluronic acid solutions, chitosan, and their mixtures as a polyelectrolyte solution. The effect of mixing ratio of two polymers and their concentrations on the amount of zeta potential were investigated in order to determine the concentration and mixing ratio with the highest interaction between the two polymers. Among the nylon monofilament yarns, layer by layer coated with chitosan and Hyaluronic acid solutions with different concentrations and mixing ratio 2, coated sample with chitosan and hyaluronic acid with concentrations of 4 and 8 (% w/v) respectively was selected as optimum sample. The comparison between properties of CH/HA coated sample and commercial nonabsorbable polyamid 6 suture revealed that the coating leads to increasing in moisture absorption and decreasing in frictional coefficient for nylon monofilament yarn from 2.5% and 0.4 respectively to 12.79 and 0.26. Also, chitosan coating in comparison to CH/HA coating leads to decrease surface roughness from 479 nm to 164nm. The comparison CH/HA coated sample with tenacity of 32.46 cN/tex and suitable knot security to commercial nonabsorbable polyamid 6 suture with surface roughness and tenacity 229nm and 39.89 cN/tex respectively showed that coated nylon monofilament can be used as an eye suture. Also, two cationic and anionic dyes were used as two drug models to investigate the release behavior of coated monofilament yarn. The results showed the coating with chitosan or HA individually, lead to release 80% and 0% the same and the oppositely charged loaded components respectively after early 24 h. The release of the both component from CH/HA coated sample was 70-80% in longer time (1 week) that showed positive effect of polyelectrolyte coating on controlled release. The results showed the Fickian release mechanism, Higuchi and first order release kinetics for coating layer in the presence and absent chitosan respectively. Considering achieved results, preparation of nanofibrous yarn from chitosan/Hyaluronic acid was done. Investigation of suitable concentration and solvent system as well as condition affecting on electrospinning of chitosan using Taguchi test showed successfully fabrication of chitosan nanofibers and unsuccessfully fabrication of pure hyaluronic acid due to low molecular weight. So, mixture of chitosan and Hyaluronic acid electrospinning was performed in different ratios. Finally, pure chitosan nanofiber yarn, chitosan electrospun nanofiber yarn sprayed with Hyaluronic acid and chitosan and hyaluronic acid blended nanofiber yarn were produced. The properties of produced nanofiber yarn and commercial Polyglactin 910 as an eye suture were compared. The results showed that nanofiber yarn produced by blended chitosan and Hyaluronic acid had tenacity of 6.65 cN/tex and roughness of 2.01 ?m in comparison to commercial suture with tenacity and surface roughness equal 4 cN/tex and 2.78 ?m respectively. The suitable rate of degradation, non-toxicity, antibacterial properties against gram negative and gram positive bacteria as well as appropriate knot security of produced nanofiber yarn indicated its ability in medical application including eye sutures.