Zinc nutrition and plant genotype are two factors that may affect the tolerance of wheat to root-rot diseases. The aim of this study was to determine the Zn status effect of six wheat genotypes on infection by F. solani . A greenhouse experiment with five bread wheat genotypes ( Triticum aestivum L. cvs. Rushan, Kavir, Cross, Pishtaz and Falat), and a durum wheat ( Triticum durum L. cv. Yav79), consisting of two levels of Zn (0 and 2 µmol L -1 , in the form of ZnSO 4 ), and two F. Solani infection level (0 and 10 6 spore/ml) was conducted. After plant harvesting, shoot and root dry matter yield were determined for each pot. Shoot and root concentrations and content of Zn, Fe, Mn, K, and Cu were measured. After plant harvesting, the severity of F. solani infection was assessed in terms of the number of roots with at least one pathogen lesion. In addition, root cell-membrane permeability and reactive sulfhydryl groups were analyzed. Zinc deficiency resulted in significant decreases in shoot dry and wet matter production of wheat genotypes, The relative sensitivity of wheat genotypes to Zn deficiency was in approximate order Kavir Cross Rushan Yav79 Pishtaz Falat in the presence of F. Solani and order Yav79 Kavir = Cross = Rushan Pishtaz Falat in the absent of F. Solani . Zinc deficiency resulted in significant decreases in shoot high. Root membrane permeability of wheat genotypes was lower in the treatments that included Zn addition than in the treatments that did not include added Zn. The presence of F. Solani significantly decreased the shoot dry matter yield of Yav79, while not other wheat genotypes affected. Root membrane permeability of wheat genotypes was lower in the treatments that included Zn than the Zn-free treatments. Zinc deficiency caused a decrease in reactive SH groups in the roots of wheat genotypes, particularly Cross genotype. Reactive sulfhydryl groups in the root decreased with the Fusarium infection. Zinc application sharply reduced the total amount of K and Mn in the shoot while increased the shoot Zn content. Application of Zn had a positive effect on tolerance of wheat to F. solani root-rot disease. This effect was dependent on Zn-efficiency of the genotypes.