Wheat makes the major staple food of people in many countries including Iran . Therefore, grain quality of wheat is of great importance to human health. In fact, it would be possible to prevent human malnutrition via improving quality of wheat grain. Biofortification is one of the most modern approaches to improve quality of agricultural products. Biofortification consisted of genetic improvements and agronomic practices. Optimum fertilizer management is one aspect of agronomic biofortification. This approach has recently been considered by researchers for improving Zn nutritional status of food crops and in turn, human. This study was carried out to investigate the effect of various zinc fertilizer treatments on distribution of zinc in different tissues of wheat genotypes ( Triticum eastivum L.) differing in Zn-efficiency. In addition, grain yield response of wheat genotypes to different Zn fertilizer treatments was investigated. Three field trials with spring and winter wheat genotypes at two locations were conducted in 2006-2007 and 2007-2008 growing seasons. Considering the critical deficiency level for the DTPA-extracted soil Zn (2 mg kg -1 ), the soils were severely deficient in available Zn. In trial 1 (in 2008), ten spring wheat genotypes were cultivated in Isfahan and Neishaboor and exposed to five zinc fertilizer treatments (no added Zn, soil application of ZnSO 4 , foliar spray of ZnSO 4 , foliar spray of Omex1 and foliar spray of Omex2). In trial 2 (in 2008), ten winter wheat genotypes were cultivated in Mashhad and Jolg-e-Rokh and exposed to four zinc fertilizer treatments (no added Zn, soil application of ZnSO 4, foliar spray of Omex1 and foliar spray of Omex2). In trial 3 (in 2007), ten spring wheat genotypes were treated with four zinc fertilizer treatments (no added Zn, soil application of ZnSO 4 , foliar spray of ZnSO 4 at tillering stage and foliar spray of ZnSO 4 at anthesis stage). Zinc distribution in stem, leaf and grain of 10 spring wheat genotypes were measured in trial 3. In all trials, grain yield and crop response to different zinc fertilizer treatments largely varied. The efficacy of different fertilizer treatments in increasing yield and grain zinc concentration was different among locations. Different fertilizer treatments dependent on the wheat genotype, resulted in increase or decrease in yield, grain Zn concentration or both. For some wheat genotypes, Zn fertilizer treatments had no significant effect on yield as well as grain zinc concentration. Different fertilizer treatments had different effects on Zn concentrations in leaves and stems of spring wheat genotypes. Zinc distribution and its traort to leaves, stem and grain of spring wheat genotypes were differently affected by Zn