Early diagnosis of cancer and isolation of Circulating Tumor Cells (CTCs) from blood flow is one of the issues that has been studied by researchers to reduce tumor caused death. A lot of works has been done on diagnosis and isolation of CTCs by acoustic waves. Since sound is a little perturbation of steady state Navier-Stokes equations, one of the simple ways of acoustic waves modeling is using perturbations. The aim of this research is the isolation of CTCs from Red Blood Cells (RBCs) and White Blood Cells (WBCs) using acoustic waves. This simulation was performed by 4 methods (solving governing equations of acoustic waves by Carlson and Bruce force method, solving governing equations of acoustic waves by Settnes and Bruce force method, solving governing equations of acoustic waves by Gorkov force method and solving Helmholtz equations by Gorkov force method). Obtained results show that isolation of CTCs can be done using acoustic waves with efficiency of 86%. These results show that in suitable conditions, the efficiency of 100% can be reached. Different parameters are effective in separating CTCs by acoustic waves. The most important parameters are cells’ radius and density. The importance of radius of cells is proportionality of applied force by cubic power of radius. Therefore, using this method, CTCs, which have radius twice that of normal cells, can be separated with high efficiencies. The importance of density is in the definition of specific path of CTCs. Actually, lower density of CTCs in comparison to blood density propels CTCs away in microchannel due to created pressure node. In addition, higher density of RBCs in comparison to blood absorbs RBCs to pressure node in microchannel. Creation of two different paths and proportionality of applied force to cells by acoustic waves with third power of radius of cells, increase the efficiency of isolation. In this research the effect of collision of cells with each other and also with walls are not considered. In addition, it is assumed that motion and applied force to cells don’t affect fluid flow and acoustic field. Keywords: Circulating Tumor Cells, CTCs, isolation, 2D simulation, acoustic
