Nowadays , growing increasing electronic waste at the same time with advances in technology and decommissioning old technologies have been the source of many environmental problems. Printed circuit board (PCB) is one of the valuable components this wastes that contains large amounts of hazardous materials and valuable resources .So, their wastes, are considered both secondary sources and environmental polluter. In this case study, bioleaching of copper from the PCB in computer motherboards is studied using mixed culture of mesophilic bacteria. In this respect, for the first time, a low grade sulfide tails containing copper as suppliers of leaching agent in process used that in addition to copper extraction containing computer motherboards, exploited copper present in their own. In this study, which their experiments was conducted tested in shaking flask, effects of various parameters including the mixing ratio of sulfide tails to electronic waste, pH, medium type, solid percent, presence - absence of microorganisms, presence - absence of sulfide tails and microorganisms and bioleaching time for copper recovery were investigated. The results showed that the highest copper recovery at shake flask (95.7%) in a mixing ratio of sulfide waste to electronic waste 1: 1, pH=1.5, 9K medium and solids 5%. Series of experiments to dissolution copper in column with 1cm dimensions of waste PCB and indirect bioleaching column by feeding bacteria that are extracted from the reactor activator of low grade copper sulfide tailings have been 93% copper recovery was obtained within 23 days