The extraction of copper from a mixture of a Cu-Ni-Co bearing sulphidic tailing and printed circuit boards (PCBs) was investigated by bioleaching process followed by solvent extraction of the pregnant leach solutions (PLS). Bioleaching was conducted in both batch and continuous modes in 2 and 50-L stirred tank bioreactors (STBRs) using a mixed culture of iron- and sulphur-oxidizing moderately thermophilic microorganisms such as acidithiubacillus caldus , Leptospirillum ferriphilum , Sulfobacillus thermosulfidooxans , and Ferroplasma . Bioleaching was firstly carried out in a 2-L STBR at the pulp density of 10% (w/v), mass ratio of PCB to sulfidic tailings of 1 to 4, Norris nutrient medium, 15% (v/v) bacteria cultivation, at 45ْC and about 87.3% of the copper was leached. Bioleaching was also conducted in three 50-L STBRs in the same conidition with 2-L STRB and in first reactor 89.6%, 70.7% and 42.3% and in second reactor 92.8, 65.1% and 45.4% and in third reactor 92.4%, 67%, and 42.4% copper, zinc and aluminum was extracted. The process was then performed in the continuous mode in which 95% of copper was extracted. Afterward, the PLS which contained 2.85 g/l Cu was purified by solvent extraction with LIX 984N extractant diluted in kerosene. In this regards, the effect of some critical parameters namely extraction time, pH of the aqueous feed, stirring rate, A (aqueous)/O (organic) phase ratio and extractant concentration on the extraction efficiency of copper was investigated. Results showed that copper can be efficiently separated from the PLS in two stages with minimum co-extraction