In this time and age, manufacture and analysis of two dimentional materials has become the center of attention due to their superb characteristics in different areas. In this research, the features of two dimentional hexagonal nanosheet of TiO2 is more thoroughly looked into and the presence of color centers in this very special 2D material is assessed vigourously. These color centers can have great potential in different fields such as photonics which is of utmost importance. Of these capabilities, emission of single photons can be pointed out, which has excellent capacity in quantum sensors, quantum nanophotonics and quantum processing. The reason for choosing this substance is its wide bandgap which can categorize it as one of the solid state platforms for the emission of single photons. For the calculations at hand, one of the hybrid functionals is employed; for these functionals are quite precise in giving the bandgap in close accordance to the experimental value. The simulation packages used in this project are FHI-aims and VASP. These simulation packages have in them the built in use of hybrid functionals and are perfect for the chosen task. The next step taken to ensure the conclusive assessment of the procured defects in emission of single photons, is the calculation of zero phonon line energy, the difference in configuration coordinates and the formation energy for each individual color center. In addition, hyperfine tensor parameters are also given to give the reader a better chance of comparing these centers with experiment. In the end, looking back at the results, the color centers that are up to the task are introduced. These color centers are the titanium vacancy and vanadium, niobium and titanium interstitial defects. The best possible transition in these defects is a transition in niobium interstitial neutral defect with a ZPL energy of 1.85 electron volts and a difference in configuration coordinates of 0.57. This transition has been looked into more vigourously.