Lime witches’ broom is an important phytoplasma disease which is first reported from neighboring countries in Persian Gulf and subsequently distributed extensively in Iran. Detection of causal agent of lime witches’ broom ( Candiadatus Phytoplasma aurantifolia) from fruit trees, field crops, vegetables, ornamentals and weeds in Isfahan province during recent years, indicates that Ca. Phytoplasma aurantifolia is spread from host to host in central part of Iran. Despite considerable prevalence of Ca . Phytoplasma aurantifolia, there is no sufficient information regarding the correlation between its genetic diversity, host range and geographics distribution. Samples of plants showing phytoplasma symptoms including lime, alfalfa, clover, apple, peach, potato and tomato were collected from the region during summer and fall 2012. Phytoplasmas were detected in symptomatic plants by nested PCR using P1/P7 or P1A/P7A followed by R16F2n/R16R2. To 0in 0in 0pt" Key words : Phytoplasma ,Ca . Phytoplasma aurantifolia، PCR-RFLP، Peanut Witches , –Broom.
