Cytogenetic analysis is one of the first steps of breeding studies. Understanding chromosomes and their behavior is effective in successful hybridization.This study was carried out on Triticummonococcum su. aegilopoides and Aegilopscylindrica to study theirkaryotypic characteristics and meiotic behavior. Eight genotypes of T. m. su. aegilooides and eight genotypes of Ae.cylindrica, collected from west of Iran were planted in two autumn planting dates. To study karyotypic characteristics root tips of seedlings were pretreated with ?-bromonaphtalene, fixed in Lewitsky solution, hydrolyzed in 1N sodium hydroxide, stained with hematoxilin and squashed. To study meiotic behavior, when the spikes were at suitable stage of meiotic division; immature spikes were harvested, fixed in Carnoy solution, stained with hematoxilin.In karyotypic study, results of analysis of variance showed statistically significant variation in long and short arm lengths, total chromosomal length but they did not have any significant variation for other karyotypic characteristics. Variation in chromosomal length could be occurred by sample preparation method or sampling at different mitotic stages. Loss of significant variation in mostkaryotypic variation is reasonable because of investigating one species. All of genotypes of Ae.cylindrica 0in; MARGIN: 0in 0in 0pt 14.2pt" Key words Karyotype, laggardchromosome, chromosome stickiness and bridge, B chromosome,asynchrony, micronuclei, meiotic index.
