In the first part of this project, a series of novel compounds based on s-triazine was synthesized by multistep reactions. The compounds were obtained by reaction of cyanuric chloride with aniline. The prepared 4,6-dichloro- N -phenyle-1,3,5-triazine-2-amine (1) was derivatized by their reactions with various aromatic amines, including 4-chloroaniline, 4-methoxyaniline, 4-aminobenzenesulfonamide, 4-aminobenzonitrile, aniline, and 4-nitroaniline. The product of the above reaction was allowed to react with hydrazine to prepared N 2 -(4-arylamino)-6-hydrazinyl- N 4 -phenyle-1,3,5-triazine-2,4-diamine (3a-3f). Subsequently, mentioned compounds were derivatized by their reactions with 2-methyl-4 H -benzo[ d ][1,3]oxazin-4-one to obtain 3-((4-((4-(arylamino)-6-(phenyleamino)-1,3,5-triazine-2-yl)amino)-2-methylqunazolin-4(3 H )-one compounds (5a-5f). Newly synthesized compounds have been characterized by FT-IR, 1 H NMR, 13 C NMR and elemental analysis. In the next part of this research, the antimicrobial screening of the title compounds (3a-3b) and (5a-5b) was examined against Gram-positive bacteria (Staphylococcus aureus, Listeria monocitogenes, Bacillus subtilis), Gram-negative bacteria (Escherichia coli, Pseudomonas aeruginosa, Salmonella entritidis ) and fungi (Candida albicans) using the serial broth dilution method. the Compounds containing cyanide, sulfonamide and chloride ( 3d, 3b, 5b, 5d and 5a) exhibit significant antimicrobial activity on several strains of microbes. The docking calculations reveal that all of the compounds show a strong interaction with the enzyme of microb indicating an effective antimicrobial activity.