Conservation planning is concerned with the optimal application of conservation actions to promote the persistence of biodiversity in situ.one of the critical stages in conservation planning is gap analysis.this analysis identifies priority sites for conservation through ecological evalouation and identifying threats to current protected areas. Threat assessment and incorporating it into conservation planning is an issue that in Iran, little attention has been drawn to it and most conservation actions focuse on ecological values of sites. So in the first part of this study, we conducted gap analysis with the aim of thread assessment using Environmental RiskSurfase Model (ERS) for Isfahan province. First, the required maps including location of mines,industries and roads were prepared and were used as risk elements.then to determine the overall contribution of each activity ,based on literature and expert opinions, variables including:weght,intensity,influence distance and distance decay function were assinged to each activity.results of ERS model revealed that west and central parts of Isfahan province are critically impacted by human disturbances, mainly because of high intensity of industries and roads. To incorporate the result of threat assessment into conservation planning, the ERS model and protected area map were overleid.Results indicated high level of intensity at some of the most important protected areas e.g. Kolahghazi national park and Mooteh wildlife refuge,calling for emergency conservation actions.Considering the results of this threat assessment and also ecological evalouation conducted previously, an area at the north-west of Isfahan, located between Mooteh and Ghamishloo wildlife refuges was selected as the study area for the second part of this study, to identify migration corridors for vulnerable species including goitered gazelle ( Gazelle subguttorosa subguttirosa ) and wild sheep ( Ovis orientalis isphahanica ).To identify corridors two modeling approach were used, Least-cost modeling and circuit theory. Using least-cost modeling, for each target species, three corridors were identified, considering their length and area, two first ones were selected as migration corridors and the third corridors (widest and largest ones) were recommended as Population Assisting Links (PALs).results of circuit theory were current maps showing the movement probability of target species across the study area. Comparing current maps of target species revealed that the level of functional connectivity for wild sheep is higher comparing the goitered gazelle. Therefore there is more opportunity for this species to migrate between wildlife refuges. Furthermore, based on current intensity, area important for connectivity was identified. We conclude that due to the vulnerability of connectivity in the study area, its protection is crucial. Comparison of results of two models used in this study indicated that areas with the high probability of movement identified through circuit theory were also fully supported by least cost corridors. But the advantage of circuit theory, over least cost modeling in this study was in evaluating functional connectivity and identifying area important for connectivity which was not achievable using least-cost modeling. Keywords: Gap analysis, Habitat suitability model, Resistance layer, Circuit theory, Current map, Migration corridor