Due to the increase of various cancers among human societies and mortality during recent years, different research groups have been working to prevent the growth of this phenomenon by using early and cheap detection techniques. On the other hand, further growth of modern science in interdisciplinary fields, micro and nano scale and their importance in different fields and applications, simplifying and performing complex experiments in microchips with high precision in the microfluidic field, are helping in cancer treatment and prevention field. Nowadays, the invention of new and effective methods and existing method promotions aimed at the treatment of cancer have been taken into consideration; one of these methods is the study of circulating tumor cells. The importance of using these methods is the possibility of diagnosis in the early stages of cancer, timely prevention of cancer progression and appropriate treatment choice. The main goal of this thesis is simulation of cell culture medium in the microfluidic chips with cells microenvironment in chemical signaling field (the chemical concentration gradient) and observation cells migration behavior in this field. During this thesis, initially, all designed steps were performed to produce microstructure as a microfluidic system, then the effect of stem cell secretome on skin cancer was investigated. Keywords: Micro, Nano, Microfluidic, Cancer treatment, Cancer prevention, Chemical gradient, Lab on a chip, Cells migration, Stem cells