This experiment was carried out to investigate the effects of curcuma longa powder on performance, egg quality, egg yolk cholesterol, fatty liver indices, the activity of some blood enzymes, serom parameters and immunity responses in laying hens fed diets with different energy levels. A total of 160 fourty-eight week-old laying hens (Hyline-36 white) were randomly allocated in a factorial arrangement based on a completely randomized design consistinged eight treatments with four replicates and five samples per replicate. Tere were four levels of curcuma longa (0, 0.2, 0.4 and 0.6%) and two levels of metabolisable energy (2750 and 2950 kcal/kg). Experiment lasted for seventy days. Feed and water were provided ad libitum during the experiment. Evaluated traits were egg production, egg weight, egg mass, food consumption, food conversion ratio, for the first 35 days, the second 35 days and also whole trail period. At the end of the first and second periods, egg quality traits were evaluated in order to investigate immune response, two birds per replicate were administered intramuscular injection with sheep red blood cell (SRBC), twice with 10 days interval, and then blood samples were collected 6 and 12 days after first and second injection, respectively. At the end of experiment, 2 hens of each cage were sacrificed for studying histological changes in duodenum. Using different levels of curcuma longa showed decrease ( P 0.05) in food consumption and feed conversion ratio, significant decrease ( P 0.01). in relative weight of liver and in egg cholesterol, significant decrease ( P 0.05) in aspartate amino transferase (AST), alanine amino- transferase (ALT), significant reduction ( P 0.05) in ratio of heterophil to lymphocyte (H:L), significant reduction ( P 0.05) in the ileal E. coli enumeration, significant improve ( P 0.05) in shell thickness, shell hardness and Internal egg quality, significant increase ( P 0.01) in relative weight of pancreas and spleen, significant increase ( P 0.05), in antibody titer against SRBC at primary immune response, significant increase ( P 0.05) in goblet cell numbers and crypt depth, villus height increased numerically, increased the kupfer cell in liver and significant increase ( P 0.05) in egg productio ratio, egg weight, egg mass, without any significant differences on other traits. Although using different level of metabolisable energy caused the significant increase ( P 0.05) in triglyceride in blood serom, significant increase ( P 0.01) in antibody titer against SRBC at primary immune response, significant increase ( P 0.05) in egg production ratio, egg weight, egg mass, significant decrease ( P 0.05) in food consumption and food conversion ratio, significant improve ( P 0.05) in shell hardness and yolk color but it had no effects on other traits. Interaction of different levels of curcuma longa and two levels of metabolisable energy had significant effect ( P 0.05) on AST and ALT, significant effect ( P 0.01) on the relative weight of liver and spleen, antibody titer against SRBC at primary immune response and food consumption, feed conversion ratio,egg production and egg mass. Also it has significant effect ( P 0.05) on the ileal E. coli enumeration, ratio of heterophil to lymphocyte (H:L) and in shell thickness and shell hardness. Keywords: Laying hens, curcuma longa, Performance, Egg quality, Immune responses