Increasing industrial activities during the recent years, followed by damping of different organic and inorganic contaminants such as copper in to the soils, is one of the problems threatening human health. High contents of copper in plants prevent their growth and interfere with major metabolic processes such as photosynthesis and respiration. Phytoremediation is a new technique through which the heavy metals from contaminated soil is taken up and accumulated in plant tissues. This method is a nondestructive and a relatively low cost technique. There are reports on using of chelating agents such as di-ethylene tri-amine penta acetic acid to increase the mobility and thus accumulation of heavy metals such as lead, cadmium, chromium, copper, nickel and zinc in Indian mustard and sunflower. Therefore at this work the effects of DTPA at three levels; control, 2.5 and 5 mg per liter of irrigation water, arbuscular mycorrhiza and mycorrhiza plus 2.5 and 5 mg DTPA combinations were investigated on copper phytoremediation by sunflower and pepper in a natural contaminated soil from Sarcheshmeh copper mine. The experiment was conducted at IUT research greenhouses in a factorial design with three replications. The results showed that DTPA and arbuscular mycorrhiza treatments enhanced copper uptake in sunflower and pepper, respectively. The DTPA-mycorrhiza combination increased copper uptake in both plants. The DTPA treatment increased copper uptake by sunflower. In general, we found that among these treatments, DTPA was the most effective treatment for copper phytoremediation by sunflower, followed by combined treatment and arbuscular mycorrhiza. The most effective treatments for phytoremediation by pepper were mycorrhiza, combined and DTPA treatments, respectively. Leaching potential index of Cu for both plants was decreased by all the treatments and the lowest leaching potential index was related to DTPA treatment. According to the results, between the two plants, sunflower showed a higher potential for copper phytoremediation in the studied contaminated soil. Keywords : Cu, Phytoremediation, Pepper, Sun flower, Arbuscular Mycorrhiza, DTPA