Pomegranate is a non-climacteric fruit with low-breath-rate and its quality is of high importance ecause of its export value. There are different ways to improve the maintenance of this fruit in cold storage. One of the modern methods is using high voltage electric field. In this research, mechanism of the effect of this field on increasing the storage life of the fruit was studied. Two copper plate electrodes with a 9 cm electrode spacing and 13.5 and 27 kV voltages were used to create the electrostatic field. The treating conditions were so that the complete pomegranate in a cold storage room with a temperature of 4± 0.5 ° C and a relative humidity of 90 ± 5% was going under a high voltage electric field for 2 hours per week, and the corresponding tests were done once every two weeks for a period of 60 days. Qualitative characteristics included weight loss in complete fruit, L * and a/b indexes in pomegranate juice and electrolyte leakage in fruit skin. The biochemical investigation of the mechanism of aril under the electrostatic field was also performed in two sectio the mechanism of non-enzymatic antioxidants and antioxidant enzymes. The results showed that high voltage electric field an increase of weight loss rate, skin electrolyte leakage, and L * index of pomegranate juice. There was no significant difference between the redness index of the sample in control group and the treated samples. The results of the biochemical mechanism indicated that antioxidant enzymes and non-enzymatic anti-oxidants were have increased power against stress, and a field with a power of 1.5 kV/cm can help in the relative maintenance of this power untill the end of maintenance. However, the results obtained from field samples with a power of 3 kV/cm were acceptable till the end of the first month, but in the second month, due to tissue damage, the values obtained were close to the control values. Also, the amount of browning in control samples and samples treated with 27 kV voltages was higher than those treated with 13.5 kV, due to the activity of polyphenol oxidase and guaiacol peroxidase enzymes. By examining the results, it can be concluded that the high voltage electric field, in addition to the ability to reduce respiration rate during storage in cold stores, has been able to influence the biological activity associated with the ROS inhibitor enzymes and by removing reactive oxygen species, can delay aging of the fruit tissue and prevent further damage caused by the stress to the tissue, through balancing ROS types and defense mechanisms. Keywords : Pomegranate storage life, High voltage electric field, Antioxidant enzymes, Non-enzymatic antioxidants