Due to accumulation of heavy metals in soil and their risk to human health, their recognition, origin and distribution have attracted considerable interests in recent years. Pollution of soil significantly reduces the environmental quality and affects the human health. Chemical approaches for evaluating soil pollution are difficult, expensive and destructive, otherwise recently methods dealing with magnetometery which are quick, inexpensive and non-destructive have been proposed as a proxy for assessing the heavy metal pollution of soil. The objective of this study was to determine the effects of land use and parent material on the spatial distribution of topsoil magnetic susceptibility and concentration of selected heavy metals in Isfahan district. A total of 204 topsoil samples (from six depths 0-5, 5-10, 10-15, 15-20, 20-25 and 25-30 cm) were taken from an area of about 5100 km 2 . Location of the sampling points was determined using GPS and their land uses were recorded. Also parent material of samples was determined by the use of geology map. The magnetic susceptibility (?lf and ?hf) and concentration of total iron (Fe), zinc (Zn), copper (Cu), manganese (Mn), nickel (Ni), cobalt (Co), lead (Pb) and chromium (Cr) were measured in the soil samples. Frequency dependent magnetic susceptibility (?fd) also was calculated by the ratio of magnetic susceptibility in low and high frequencies. Selected soil chemical and physical properties including pH, electrical conductivity, organic matter, calcium carbonate equivalent, sand, silt, clay and gypsum were also determined to understand the relationships between magnetic susceptibility and soil properties. Spatial distributions of studied parameters were explored produced using kriging method. Generally, six different types of vertical distribution magnetic susceptibility patterns observed in the studied area, while patterns were indicators of soils developed on igneous and sedimentary rocks in agricultural, rangeland and urban land uses. It appears that the concentration of Pb, Zn and Cu has been affected by anthropogenic sources such as traffic emissions and mining activities, while Fe, Mn, Ni and Co might be attributed to both lithologic and anthropogenic sources. Low value of heavy metal concentration and significant correlation among them, in un-urbanized area confirmed that almost all elements showing natural sources and didn't have anthropogenic enhancement. The results showed that concentration Mn and Co positively correlated with ?lf and ?hf in unurbanized area. In urban area the concentration of Fe, Zn, Mn, Ni and Pb showed negative significant correlations with ?fd, while in unurbanized area Fe, Zn and Ni concentrations showed positive significant correlations with ?fd, which indicated the magnetic properties of the samples are predominately contributed by super-paramagnetic particles. High concentration of Pb, Zn and Cu in urban area showed that these areas has been polluted compared to agriculture and pasture land uses. Results showed that two major factors controlling the enhancement of magnetic susceptibility and heavy metals are lithologic and anthropogenic influences in the study area. There were negative significant correlations between electrical conductivity, gypsum, calcium carbonate equivalent and organic matter with magnetic parameters. Increasing of soluble salt, gypsum, calcium carbonate equivalent and organic matter decreases the magnetic susceptibility of soils by dilution effect of magnetic minerals. Basically in this investigation, our results indicated that pattern of magnetic susceptibility in different parent material provide a significant tool for interpretation of magnetic susceptibility in different land uses. Also, magnetic susceptibility could be used for assessment of heavy metal pollution in soil. Keywords : Distribution of magnetic susceptibility, Land use, Heavy metal, Urban and industrial pollutions, Parent material