The present study was carried out to determine the best pre-sowing 15 different treatments for seed germination in Allium jesdianum ( A. jesdianum ). The highest seed germination (58.33%) was observed at chemichal treatment including NAA (1 mg/L) + BAP (3 mg/L) of hypocotyl explants on Murashige and Skoog (MS) media. The highest callus induction (86.7% ) was recorded at naphthalene acetic acid (1 mg/L) + benzyl amino purine ( 3 mg/L)which was supported at MS/2 media, among diffferent combinations of plant growth regulators. Two elicitor including methyl jasmonate (MeJ) ( 0, 25, 50 and 100 µM ) and Putrescine (0, 0.5 and 1 mM) were used to investigate their effects on lipid peroxidation, callus growth, total phenolics content (TPC), the content of total flavonoid (TFD), total flavonols (TFL), Anthocyanin (Ant), chlorophyll, carotenoid and antioxidant activity through DPPH (1,1-diphenyl-2-picrylhydrazyl) method under callus culture. The results showed the superiority of MeJ over Put for the content of secondary metabolites and antioxidant activity in callused of A. Jesdianum . The highest TPC ( 6.02 mg GAEg -1 FW) TFD ( 0.52 mg QEg -1 FW) TFL ( 0.39 mg QEg -1 FW) were observed at 50 µ M of MeJ, but the highest value for anthocyanin ( 8.99 µ mol g -1 FW) was achieved at 25 µM of MeJ. The maximum DPPH activities were observed at 50 and 100 µ M of MeJ. utrescine (0.5 mM) elicitation showed only superiority for callus growth rate (0.53 mm/day) and carotenoids (0.045 mg g ?1 DW). Thus, new protocols have been developed for seed germination, callus induction and the elicitors-induced enhancement of metabolites and antioxidant activity in callus culture of A. Jesdianum .
