Heavy metals soil pollution is one of the environmental problems have attracted the attention of many investigators. Heavy metal pollution in addition to the harmful effects on crop yield and quality, ultimately endangers public health and other living organisms. Therefore, soil removal of these toxic metals is quite important. Phytoremediation is one of the methods used in recent decades to refine the polluted soils that given plants used to remove heavy metals from the contaminated soil. Salinity is an important factor affecting metals uptake of plants increasing the solubility of the metals. Mycorrhizal fungi also influence the heavy metal uptakes of plants. These fungi by developing a widespread hyphae system into the soil help water and nutrients absorption and therefore, increases plants resistance to various environmental stresses such as salinity, heavy metal contamination and drought. Canola is a plant that in addition to salt tolerance has the ability of heavy metal accumulation in its tissues. Therefore, in this work, the effects of mycorrhizal fungi and salinity on the heavy metals uptake of canola was investigated using a factorial completely randomized design with two factors in greenhouse of college of agriculture, Isfahan University of Technology. The first factor was fungi in three levels; absence and presence of Glomus intraradices and Glomus mosseae species and the second factor was salinity with three levels; as EC of irrigation water as 0.5 (tap water as control), 3 and 6 dS/m of natural saline water. The experimental pot soils were inoculated with propagules of fungi, then the plant seeded and up to 3-4 leaf stage, the pots were irrigated with tap water. Then the salinity treatments using a natural saline water and considering a leaching fraction of 15% applied for 45 days. The irrigation was carried out at 50% of field capacity. A month after salinity application, the antioxidant enzymes catalase and peroxidase activities were measured. Plants were harvested 70 days after planting. Results showed that salinity and mycorrhiza inoculation increased plant growth parameters, while inoculated plants had a better performance than the controls. Salinity increased Na, Ca, Cl and heavy metals Pb, Zn, Ni and Cu concentrations of plant tissues. Mycorrhiza inoculation also increased Pb, Zn, Ni, Cu and Ca, but reduced Na concentrations. Mycorrhiza inoculation increased P concentration particularly in salinity stress conditions while, salinity showed an adverse effect. Mycorrhiza inoculation and salinity increased Pb and Zn Translocation from root to shoot. Zn in root and shoot showed a relatively high concentration, while regarding the translocation factor this element was in an average condition. Following Zn, Pb content of the plant tissues was also relatively high. Apparently, due to the high concentration of lead in roots, translocation of this metal to shoots was low showing the minimum translocation factor. While, the Ni translocation factor was relatively high especially in non-inoculated plants and 0.5 salinity level. But salt and mycorrhiza inoculation both decreased Ni transfer to shoot. In average this metal showed the highest translocation factor among the metals. Cu concentration similar to nickel was relatively low in roots and shoots, while its translocation factor was similar to Zn. Mycorrhiza inoculation and salinity increased catalase and peroxidase enzymes activity. Catalase activity was more affected by mycorrhiza inoculation, while salinity was more effective on peroxidase activity. Glomus intraradices showed better effects on plant growth and nutrient and metals uptake over Glomus mosseae representing a more effective symbiosis of this species, especially in stress conditions. Key Words Heavy Metal, Phytoremediation, Salinity, Mycorrhizal fungi, Glomus intraradices , Glomus mosseae