An experiment was conducted at the Isfahan University of Technology, Iran, Isfahan (40° 85' N, 77° 83' W) during September to November 2009 in order to evaluate the effects of mycorrhizal symbiosis on the response of safflower genotypes to saliniy. The pot experiment was laid out using a factorial arrangement in a randomized complete block design with four replications. In this experiment the inoculated and uninoculated plants of four safflower genotypes (Kermanshah 47, Hamadan 17, Dincer, PI536636) were tested at four salinity levels (0, 50, 100, and 150 mM of NaCl). Plant height, number of leaves per plant, leaf area per plant, plant dry matter, chlorophyll content, chlorophyll florescence, net Co2 assimilation, stomata conductance, the concentration of sodium, potassium, and phosphorus in roots and leaves were measured. With an increase in salinity level plant height, leaf number, leaf area, plant dry matter, chlorophyll content, photochemical efficiency (Fv/Fm), net Co2 assimilation, stomata conductance, the concentration of potassium and phosphorus in roots and leaves were reduced and the concentration of sodium in plant tissues was increased. The interaction between salinity levels and genotype was significant on plant height and leaf area per plant but it was insignificant on plant dry matter. The effect of salinity was not significant on the ratio of shoot/ root dry weight. The inoculation of plant roots with mycorrhiza led to an increase in the number of leaves per plant, leaf area per plant, plant dry matter, chlorophyll content, photochemical efficiency, net Co2 assimilation, stomata conductance, the concentration of phosphorus in plant tissues. Mycorrhizal inoculation had no significant effect on the ratio of shoot/ root dry weight. The interaction between salinity levels and mycorrhizal inoculation was significant on the concentration of sodium in the roots, potassium/ sodium ratio in roots and leaves, photochemical efficiency, the content of chlorophyll A, plant height, number of leaves per plant, leaf area per plant and leaf dry weight. As the salinity level was increased up to 50, 100, and 150 mM, leaf dry weights of inoculated plants were decreased about 12, 25, and 24 percent respectively. These decreases were about 13, 27, and 57 percent for uninoculated plants. The results from this experiment show that salinity can negatively affects different growth parameters of safflower genotypes and these effects can be mitigated with mycorrhizal inoculation.