Pistachio nut tree is one of the crops which has drought tolerance and traditionally been cultivated in different areas of Iran. Soil and water salinity, water relative deficiency in arid and semi-arid areas are limiting factors which have restricted acreage of pistachio cultivations. Reduced rainfall and low water availability for agriculture in recent years has led to carrying out research on the use of plant growth regulators to improve drought tolerance in plants. The present study was performed to investigate the effects of salicylic acid and methyl jasmonate at different concentrations (each of them at four levels) on enhancing drought tolerance in ghazvini pistachio seedlings under in vitro conditions. Various levels of water stress were induced using five concentrations of agar (8, 10, 12, 14 and 16 gl-1) in MS medium. Water potential of different media were -0.77, -0.84, -0.91, -0.98 and -1.05 Mpa, respectively. The results of the study indicated that growth indicies such as number of leaves, shoot and root fresh weight significantly decreased under different levels of water potential. However, water stress had no significant effect on shoot length. The maximum number of leaves and fresh weight of shoots were obtained from methyl jasmonate at 50 µM. All concentrations of salicylic acid decreased the number of leaves, shoot and root fresh weight. SA had no significant effect on shoot length. There was a significant increase in proline content at -1.05 Mpa water potential and the highest rate of proline was related to the use of 0.3 mM salicylic acid although no treatments of methyl jasmonate had significant increase in proline content. Increasing the water potential of the medium leads to a decrease in chlorophyll a, b, a + b and the carotenoid. Application of 200 µM methyl jasmonate led to the highest increase in all chlorophyll contents. The highest amount of carotenoid was achieved using methyl jasmonate at 50 µM. All treatments of salicylic acid reduced chlorophyll pigments and carotenoid. Malondialdehyde rate was not affected by water potentials and methyl jasmonate. Salicylic acid was increased, leading to an increase in malondialdehyde. The activity of antioxidant enzymes catalase, peroxidase and ascorbate peroxidase increased with increasing the water potentials. The highest catalase activity was related to the use of methyl jsmonate at 200 µM and salicylic acid at 0.1 mM. Using 0.3 mM Salicylic acid caused the highest enzyme activity of peroxidase and ascorbate peroxidase. According to the results of this study, it could be concluded that the use of 50 µM methyl jasmonate resulted in improving pistachio status under water potential stress. Also, the use of high concentrations of salicylic acid caused pistachio susceptibility to drought stress.