Over the decades, pressure exerted by natural and artificial selection could have progressively reduced the genetic diversity of the wheat germplasm. Breeders seek to study older types of wheat to transfer lost genes such as resistance to biotic and non-biotic stresses to modern cultivars. A very old type cultivar of wheat, has been crossed with a modern cultivar and then a double haploid (DH) population comprising of 151 lines was developed. In this study, in order to evaluate the genetic diversity and to select the drought-tolerant introgressin lines, 151 DH lines along with two parents and four controls were evaluated under two moisture conditions (non-stress and drought-stress) according to a simple lattice design in field. Considerable gebnetic variation was observed among DH lines for most of the traits indicating that selection for these traits can be effective to develope lines with higher performance or specific purposes. The results showed that drought stress had significant effect on all the traits except of the relative water content of leaf. For example, drought stress reduced grain yield by 40%. The trait lodging had the highest percentage of reduction due to drought stress (74%). Transgressive segregation was observed for most of traits indicating the possibility of selection superior lines. In both moisture conditions (no stress and drought stress), the trait awn length had the highest (49%) narrow sence heritability and the trait relative leaf water content had the lowest one (12%). Results of correlation analysis indicated that traits harvest index, biological yield, number of spikes, number of seeds per spike and 1000-seed weight had positive and significant correlation with grain yield. These relationships can be used to develop selection criteria fo indirect selection in wheat improvement programs. According to the results of tolerance indices and biplot of principle component analysis, specific and superior lines were identified. For example lines numbers 184 and 45 had the highest drought tolerance and yield stability under drought condition. Also lines 7, 139 and 188 introduce as early flowering with high grain yield for future study. Keywords: Genetic bottelneck, Double haploid , Narrow sence heritability, Transgressive segregation, Drought stress