In order to determine the effects of different levels of Amet protein binder (0, 1.5, 3, 4.5 and 6%) in substitution for fish meal on performance and digestibility of nutrients, an experiment was conducted using 400 broiler chickens (Ross strain) with 5 treatments of 4 replications. The experiment was performed in a completely randomized design. Weight gain, feed consumption, and feed conversion ratio were determined at the end of each period of experiment. In addition, the digestibility of nutrients and digestible energy (DE) of whole diets were measured by ileal collection method. The results showed that treatment contained 6% Amet decreased feed consumption at the starter period (p 0.001), other treatment had not significant different in this period. Levels of 4.5 and 6% Amet significantly decreased feed consumption in grower period (p 0.001). Treatments had not effect on feed consumption in finisher period. Feed consumption was higher for 1.5, control and 3% Amet in diets during whole period, respectively. Weight gain decreased only for treatment contained 6% Amet at the starter period. At grower period, treatment contained 1.5% Amet was showed greatest weight gain versus other treatments, but this different had not significant versus control treatment. Addition 1.5% Amet versus control treatment insignificantly increased weight gain in all periods of the experiment. In the starter period, 1.5% Amet improved feed conversion ratio (p 0.001). The 6% and 4.5% Amet in all periods of experiment and the finisher period increased feed conversion ratio, respectively. Treatment contained 1.5% and 3% Amet increased protein efficiency ratio (PER) at the starter period. In the grower period PER was no significantly different except treatment 6% Amet. Treatments contained 4.5 and 6% Amet decreased PER at the finisher period. Relative weight of intestine and percent of abdominal fat were not significantly affected by treatments. Treatment with 6% Amet increased the relative weight of liver (p 0.05). Replacement of different levels of Amet except the treatment contained 6% Amet had not significant effect on percentage of carcass. Meanwhile, treatment didn’t affect digestibility of ash and DE. The level of 1.5% Amet versus 6% Amet significantly increased digestibility of protein (p 0.05). The levels of 0, 1.5, and 3% Amet versus 4.5 and 6% Amet significantly increased digestibility of fat (p 0.001). It seams that inclusion of 1.5% Amet binder in the diet can positively affect performance and DE of diet, also provide apart of requirement of protein in broiler chickens.