Iran is one of the centers of genetic diversity for cultivated and wild safflower species. The most important insect pest of safflower in Asia and Europe is safflower fly, Acanthiophilus helianthi Rossi, which could stop the safflower growth and head production. In order to evaluate resistance or sensitivity of different safflower genotypes to safflower fly, an experiment was carried out in a randomized complete block design with 12 treatments and three replications at the research farm of Isfahan University of Technology. The tested genotypes were three cultivated genotypes, Ac-stirling, C 111 and C 4110 , from Carthamus tinctorius and five wild genotypes, Azari, Arak, Shiraz, Hamedan and Lavark from C. oxyacanthus , and three genotypes from other wild species of C. lanatus , C . dentatus and C. palaestinus , and one hybrid genotype (C 111 × Isfahan) derived from F7 breeding generation. From head setting until harvest four times of sampling using 20 selected heads in each plot were carried out. Several parameters including percentage of infested heads to different stages of safflower fly, larval and pupal weight, male and female longevity and percent of parasitized pupae were assessed. At harvesting time the percentage of infested seeds and heads and total number of larvae and pupae were evaluated. The results of both years showed that larvae and pupae reared on Ac-stirling genotype had the highest weight. In total, this genotype had the highest percentage of infested seeds and heads. In both years, usually a higher larval and pupae mortality was recorded in wild and hybrid genotypes, than in cultivated genotypes. In 2013 the lowest and highest percentage of seed loss was recorded in hybrid (1.7±0.3) and Ac-stirling (16±1) genotypes, respectively. In 2014, the lowest seed loss was recorded in C. palaestinus (0.6±0.2) C. lanatus (1.7±0.6) and hybrid (1.9±0.3) genotypes and the highest percentage was observed in Ac-stirling genotype (28±1). In the no- choice experiment four males and four females per plant were released in each cages 1.2 (width) × 1.5 (length) with a height of 1.2 m. The highest total mortality of larvae and pupae was observed in hybrid genotype in 2013 and in Azari and hybrid genotypes in 2014. This may indicate the presence of antibiosis mechanism in some wild accessions and hybrid genotype In addition, the resistance of safflower genotypes to another pest, Eublemma parva (Lep: Erebidae) was also investigated. This species has been distributed in southern Europe, to Central Asia and has been reported from safflower in Iran. The results showed that E. parva preferes cultivated genotypes to wild ones. Also, when wild genotypes were attacked, the percentage of infested seeds was low. Among tested genotypes, the most sensitive genotype to this pest was C. dentatus . Although this genotype showed a low percentage of infested heads, the highest seed loss was recorded (99±1/1). Keywords: Pest resistance, Safflower, Acanthiophilus helianthi , Eublemma parva , Choice test, No-choice test