Various methods and combinations have constantly been used in treatment and healing of wounds over the entire human history. H ydrogel dressings have shown promising results in wound treatments. Variuos properties of h ydrogels such as wound exudate absorbence , gas exchanging, water vapor exchange , preventi on of infectious agents are among advatages associated with h ydrogel wound dressings . In this study, soy protein isolate hydrogel was used for p reparation of wound dressing on a polyproplene needled non - woven substrate. The prepared dressing enjoyed the desirable properties of soy protein, and the acceptable mechanical performance of needled non - woven polypropylene fabric . Exce l lent C hamomile conc entrate healing properties were used to prepare the soy hydrogels. P roperties of the final wound dressing including mechanical properties, swelling and degradation percentage, air permeability and water vapor transmission rate, moisture absorption percenta ge, water contact angle and horizontal wicking time, antibacterial activity and cytotoxicity were evalulated . It was found that combination of soy hydrogel and nonwoven polypropylene fabric has profound effect on healing performance of the prepared dressing. The results also showed the dressing has strength rating of 2.08 MPa, swelling percentage of 375%, air permeability of 1207 ml / (s.mm ^ (2)), water vapor transmission rate of g / (m ^ 2). day) 744, water contact angle of zero , horizontal wicking time of 4.5 seconds . These were considred to be within the acceptable range of commercially avilable wound dressing. Despite the use of C hamomile concentrate in the preparation of soy hydrogels, antibacterial activity was not observed . This was considred to be due t o the low purity of C hamomile concentate used. Cell culture results pointed to more intensive growth of fibroblast cells in samples containing hydrogels . This was found to be due to a positive effect of soy hydrogels on the growth and proliferat ion of fibroblast cells. Additionally cell culture experiments showed no significant difference in growth and proliferation of cells on C hamomile contained and non - C hamomile contained samples. Finally it was conculded that the prepared wound dressing sampl e can be sucessfully be used in healing of wounds.