In this research, centrifuge and electro-centrifuge spinning were introduced and their mechanism were studied and compared with conventional electro-spinning. In addition, main parameters of electro-centrifuge spinning were detected, and the their effects on the diameter of produced nanofiber and spinning process were determined. In electro-centrifuge spinning with fixed machine setting, the possible spinning range is dependent on applied voltage, solution viscosity, and rotational speed. In this study, the useful range of nanofibers production defect-less was identified and an empirical relationship has been presented to predict the maximum possible rotational speed at each concentration and applied voltage. Production capacity in electro-centrifuge spinning increased significantly in compare with electrospinning, i.e. this ratio is about twelve times for solution of 13% concentration. While, in electrospinning systems, the solution flow rate is controlled by a pump; but in electro-centrifuge spinning system the solution flow rate is dependent on machine and process parameters. In this study, effective variables on the solution flow rate were evaluated both in theory and experiment; then a general theoretical relationships were introduced to predict the solution flow rate in electro-centrifuge spinning. In the next step, the ability of electro-centrifuge spinning to produce aligned nanofibers was investigated; and image processing technique is used to evaluated the effects of influencing variables on nanofiber alignment. In the final section of this study, the nanofiber structure produced by electro-centrifuge spinning and electrospinng was investigated by X-ray diffraction. The crystallinity index and crystal size of produced nanofibers is higher for the electrospinning system in compare with electro-centrifuge spinning system. While the crystals orientation in nanofiber structure for the electro-centrifuge spinning increase in compare with electrospun nanofiber. An increase in the rotational speed of the electro-centrifuge spinning system increase the crystals orientation