Extensive research in the field of tissue engineering has been conducted in recent years, and organ destruction and repair is one of the main challenges of medical science today. Replacing damaged tissue with new tissue, it has many limitations in terms of cost and public access. Using three main factors: cell, scaffold and growth factors, tissue engineering science tries to facilitate the healing process of injuries and losses organs. Tissue engineering seeks to make living replacement parts to the body. The first point that must be considered in making the cell substrate for cell repair and growth is that ist ingredients must be completely biocompatible and not toxic to the cell, which is called cell scaffold. One of the most common methods in tissue engineering is the production of scaffolding by electrospinning method. Today, a combination of biological and traditional polymers are used to produce scaffolding. Synthetic polymers increase the strength of scaffolds and biopolymers increase cell adhesion and reduce the body's immune responses. In this study it is intended, with the help of synthetic polymer, polyvinyl alcohol (PVA) and mossilage in the root of the Iranian safflower plant with the scientific name ( Eremmurus persicres ) collected from the Khoroose-Galue paws located in Semirom city of Isfahan province, to produce scaffold and study its physical and chemical properties.