Recently, there have been so much reasearch on designing and making nanofiberous sacaffolds with different chemical and mechanical charachterstics which shows a proper bidegradbility for heart tissue engineering (HTE). In this research for designing and making a scaffold which can be used in heart tissue engineering previous biomaterials were studied and then Poly (Glycerol Sebacate)-co-polyethylen glycol was chosen to be synthesized and used in electrospining for scaffold production. Poly (Glycerol Sebacate)-co-polyethylen glycol is synthesised with 30%, 50% and 70% glycerol to PEG ratio. Fourier Trasform Infrared (FTIR) test is performed to verify the polymerization process.It’s observed that electrospining pure PGS-co-PEG polymer is not possible. For making it possible to produce the nanofiberous scaffold, the synthesized polymer is mixed with polycaprolacton (PCL) which is a popular biomaterial for tissue engineering applications with 1:1, 1:2 and 1:3 PCL to PGS-co-PEG ratio. Nanofiberous scaffold is produced through electrospinning proccess. Due to high number of tests and sample that were needed, using taguchi methode the number of required tests and samples were reduced. To test biodegradablity, scaffolds were immersed in phosphate buffered saline (). Stress-strain test is also applied to nanofiberous scaffolds and mechanical properties were studied. Measuring water contact angle is performed for hydophility test. Results show that measured cotact angles for all samples were less than 44 degrees which show high hydophility of scaffold. Elongation at break for samples was up to 160%. Generally, the most optimized Poly (Glycerol Sebacate)-co-polyethylen glycol nanofiberous scaffold was produced with 1:2 PCL to PGS-co-PEG ratio,13 wt% polymer consentration and 30% glycerol to PEG ratio. Average fiber diameter for corresponding elecrospinning condition is measered to be 250 nanometers. The scaffold had an even diameter distribution and showed about 100% elongation at break.
