: Nerve regeneration is a complex phenomenon .Central nerve system (CNS) regeneration is more complicated than peripheral nerve system as there are factors which inhibit repair. Nerve tissue engineering is one of the most developing methods to restore nerve system .One of the most important part of scaffold design in tissue engineering is polymer blending for the optimization of a biomaterial scaffold with suitable cell sources. .PCL develops the mechanical properties of the scaffold, gelatin provides good cell adhesion and proliferation and naturally derived fibrinogen protein accelerate axon migration ,decrease body immunology response and increase cell adhesion and proliferation by RGD receptors. In this Regard, Random PCL/gelatin/fibrinogen nanofibrous scaffold were fabricated by electrospinning method in 3 different weigh ratio [PCL/Gel(6:1);PCL/Gel/Fib(6:1:1/5);PCL/Gel/Fib(6:1:3)] termed as S1,S2,S3 which mimicked ECM in spinal cord region .The fiber diameter of fabricated scaffolds were obtained in the range of 75-260 nm in which fiber diameter decreased when fibrinogen content in scaffold increased. Chemical characterization of the scaffold by FTIR confirms presence of both proteins(Gel Fib) and PCL and absence of any reaction between them. The tensile strength and stiffness decreases by Fibrinogen adding from 8/11 MPa and 25/36 MPa for S1 to 2/65MPa and 21/90 for S3.Both static and dynamic contact angle of scaffolds were measured and results shows that increasing fibrinogen content in the scaffolds ,increased scaffolds hydrophilicity. Cell culture studies using Adipose Derived Stem cells(ADSCs) revealed significant higher cell proliferation on PCL/Gel/Fib(6:1:3) scaffold compared to cell proliferation on PCL/Gel/Fib(6:1:1/5) and PCL/Gel(6:1). This results demonstrates that PCL/Gel/Fib scaffolds provides suitable substrates for central nerve tissue engineering. Keywords: Central Nerve Tissue Engineering, Electrospinnig, PCL, Gelatin, Fibrinogen
