In recent years, the development of scaffolds with suitable physicochemical and biological properties is one of the most important challenges in tissue engineering. The purpose of this project is to investigate the ability of collagen extracted from the skin of rainbow trout to be used in tissue engineering. For this purpose, first collagen was extracted from fish skin and then collagen scaffold was fabricated and evaluated using freeze drying process. Then, due to poor mechanical properties and high collagen degradation rate, the synergic role of boron nitride (BN) nanoparticles as well as two step crosslinking process (hydrothermal (DHT) and glutaraldehyde (GA) crosslinking) to promote the mechanical and biological properties of collagen scaffolds was investigated. The role of cross-linking process and different concentrations of BN nanoparticles (0, 3, 6, 9 and 12 wt%) using FTIR, XRD, DSC, SEM, Compressive strength and degradation tests were evaluated. Finally, the toxicity of scaffolds on MG63 cell line was evaluated. The results confirmed the isolation of type I collagen with good thermal stability compared to other sources. In addition, the synergic role of BN nanoparticles and two-step crosslinking process resulted in a noticeable improvement in degradation rate and mechanical properties of collagen-BN scaffolds. Based on the results, incorporation of 6 wt.% BN along with two-step crosslinking resulted in increase in compressive strength (9.5 times) and elastic modulus (4
