According to the World Health Organization, in 98 countries, leishmaniasis is indigenous and over 350 million people are at risk. The number of people with Leishmaniasis is estimated at 12 million. Annually, 2 million new cases of leishmaniasis occur, of which approximately 0.5 million are diagnosed with calazar and 1.5 million patients with cutaneous leishmaniasis. Leishmaniasis is commonly transmitted by the species of mosquitoes. Despite the knowledge of the parasite that causes the disease of cutaneous leishmaniasis, the knowledge of the vector of the disease, the ways of tralantation of cutaneous leishmaniasis, and serious researches into the disease, unfortunately, cutaneous leishmaniasis is still in many countries of the world and even is expanding. leishmaniasis also exists in Iran because its carriers and its reservoirs exist in our country. The treatment methods used for this disease are divided into three methods: physically treatment (surgery and removal of the lesion, etc.), topical (glucantime injection) and systemic therapy. The therapeutic effect of the antimony compounds and amphotericin B has been proved. To reduce the side effects of these drugs, use less drug and also the ease of use, it has been considered in new drug delivery systems. Polyvinyl alcohol as a biocompatible polymer with the ability of electrospinning and nanofiber formation, has been selected as a suitable drug carrier. In this study, the use of amphotericin B as an appropriate drug for the treatment of leishmaniasis, with the possibility of loading in polyvinyl alcohol nanofibers, was investigated. Initially, 6, 6.5 and 7% solution of polyvinyl alcohol were prepared in water and the possibility of electrospinning was investigated. Then, considering the morphology of the prepared nanofibers, 7% concentration was reported as the optimum spin concentration. Subsequently, 10% of amphotericin B was added to the solution and after spinning, the analysis of SEM images taken from nanofibers showed a diameter range of 180 to 330 nm. The results of FTIR confirm the presence of the drug in the nanofibers without inappropriate interactions between the drug and the polymer. The release of the drug from nanofibers was investigated using two methods, conventional methods and using a skin simulant cell. These results showed that drug release from skin simulant cell is more controllable. The mechanism of release in both methods follows Fick’s law, and its release kinetics follow the Higuchi model in both methods. Subsequently, the nanofibers containing the drug were produce by a needleless electrospinning system, and then placed in a suitable structure as wound dressing, and sterilized by gamma irradiation. The dressings were then tested in a clinical trial test and the results of clinical trials indicated that dressing was effective in wound healing.