Wastewater reuse is one of the sustainable water resources management strategies. One of the most important environmental issues is the disposal of wastewater. Disposal of wastewater without any treatment process causes permanent damage to the natural ecosystem. Basin-fishery wastewater is one of these wastewaters that are easily released into the environment today, resulting in persistence, unpleasant odor, and contamination of surface and subsurface flows. Due to the development of aquaculture and the use of dense and ultra-dense systems, the quality of water consumed in the ponds and the effluent of the fish ponds has decreased, which is an environmental concern. Nowadays, aquaculture is considered as one of the sources of human food production. Therefore, the construction of fish ponds, and consequently, the effluent of the fish ponds, is increasing. In this study, the feasibility of treatment of wastewater in fish ponds was investigated by vertical constructed wetlands. Assessments were carried out on effluent from a fish farm with a capacity of 5-6 tons of fish per year in Varzaneh, 110 km east of Isfahan. For this purpose, two vertical -horizontal subsurface constructed wetlands were built in Varzaneh. Each wetland consisted of a vertical subsurface wetland 100 cm in width and 120 cm in depth and a horizontal subsurface wetland 450 cm in length and 75 cm in width, and 60 cm in depth. Two treatments for the porous bed were used, e. g. washed and grained pea-sand with 14-13 mm d 50 and sand-straw mixture. Both treatments were cultivated with conventional straw. The effluent was introduced into the vertical subsurface wetland by a reservoir with a discharge of 480 liters per day and then gravitationally entered into the vertical subsurface wetland. Accordingly, the vertical wetland retention time was 1 day, horizontal wetland retention time 3 days, and total wetland retention time 4 days. Samples were taken from inlet and outlet of the systems at two times and at different concentrations proportional to fish weight. Nitrate, ammonium, phosphorus, BOD and TSS were measured in the samples. The removal efficiencies of nitrate, ammonium, phosphorus, BOD and TSS for sand bed treatment were 52-67, 45-69, 28-48, 66-89, 81-94% and for the sand-straw bed were 64-64, 62-84, 33-53, 80-94, 69-91% respectively. According to Standard 8726, except for TSS, the concentration of wetlands output for other parameters is not within the standard allowed for reuse in fish ponds, and further effluent treatment is needed. According to Bulletin 535, the output concentration of the two systems for use in agricultural and irrigation applications, surface water discharge and artificial feeding is permissible. Wetlands are very good for wastewater treatment to protect and prevent environmental pollution. According to reliable research done worldwide, the performance of the present design systems is at optimum level. Key Words: Hybrid subsurface constructed wetlands, rainbow trout Wastewater, Wastewater Treatment, Varzaneh,