Recently, electrospinning technology has emerged as a versatile method to produce biomimetic non-wovenmats comprising a large network of interconnected fibers and pores.Especially, a series of electrospun nanofibers based on chitin and chitosan have gained great attention owing to their extensive biomedical applications such as tissue engineering scaffolds, drug delivery.Gold nanoparticles functionalized fibers, provid non-toxiccarriers for drug and gene delivery applications. Including gold nanoparticles in functionalized electrospinning fibers allows an effective attachment of CS nanofiber to thiol groups includind DNA-SMolecules. If Au can be integrated into CS nanofibers, it would largely expand the applications of CS in the field of biomedicine. Au nanoparticles (Au) containing polyvinyl alcohol (PVA)/chitosan (CS) composite nano?bers were successfully prepared by a simple and effective method called electrospinning.Au were ?rstly synthesized under a mild condition with CS as the reducing agent and stabilizer, followed by mixing with PVA solution andsubsequentlythe resulting ?bers were fabricated. To stabilize the gold nanoparticles in solution biocompatible polymer chitosan from various methods such as mixing chitosan, polyvinyl alcohol and mercapto propionic acid and tri polyphosphate more stabilizers were used. Two CS and PVA polymer solution with suitable weight ratio of 20/80 and different concentrations of 500, 250 ppm gold nanoparticles were prepared and electrospun. Electrospinning process in this research with voltage 20 kV, distance needle tip to collector 20 cm, flow rate 2 ml / hr was carried. Nanofiber morphology and distribution of gold nanoparticles using Scanning electron microscope (FESEM) were studied. Fiber structure and functional groups of polymers were studied by FTIR. To examine the thiolgroups bind to gold nanoparticles of mercapto propionic acid and change SH group soluble mercapto propionic acid to Au-S in the fiber was investigated by FTIR spectroscopy. The terminal carboxylemercapto propionic acid is activated by EDC and the fluorescence probe FA was connected by amino group. Distribution of fluorescence, which indicates the distribution of gold nanoparticles and Au-S bonds are evaluated by fluorescence microscopy. Finally ,the gold nanoparticles binding thiols DNA and S-Au structures were identified with FTIR. Produced nanostructured without the grain with average diameter of 198 nm .The results showed that by increasing the gold nanoparticles, the average diameter of hybrid nanofiber reduced and the particles are as well as in nanofiber. Use a variety of stabilizers for gold nanoparticles cause more particles located on the surface of the nanofibers to form a consecutive layer structure seem to beand enough space to attach particles to provide other levels.In the case of particles coated mercapto propionic acid, being coated on the surface of gold nanoparticles to prevent stick together of the particles to each other and reduce the diameter of the nanoparticles compared to other coatings. Using fluorescence microscopy dispersion and thiol compounds binding to gold nanoparticles well observed.FTIR remove a thiol group in the area in 2600 and showed Au-s bonds.The nanofibers functionalized with gold nanoparticles and DNA can be used as a sensor to accurately identify supplement DNA