Verticillium wilt disease of olive, is one of the most important diseases on olive tree considered in the cultivated areas and is found world wide. Reviews related to the biological control of Verticillium wilt using fungi as antagonists showed that only pathogen antagonists have been introduced in the laboratory and greenhouse and field assays are limited to species of genera Gliocladium, Talaromyces , Trichoderma , and Arbuscular mycorrhiza fungi (AMF). Inoculated olive seedlings with mycorrhizal fungus Glomus mosseae in soil contaminated with V. dahliae , reduced disease severity and increased the growth of olive seedlings. In this study, four species of endomycorrhizae Paraglomus occultum, Glomus etunicatum, G. fasciculatum and G. clarum isolated from rhizosphere soil of olive orchards in Guilan Province (Loshan, Manjil and Rudbar) on the developmental process of interaction between mycorrhizal fungi and Verticillium wilt of olive was evaluated. In the first stage, causal agent factor Verticillium wilt of olive trees Isfahan was isolated and purified. Based on the morphological and molecular studies, Verticillium dahliae kleba was identified. This is the first report of isolation of V. dahliae of the olive in Isfahan province. In the second stage of separation and purification of mycorrhizal fungi, using sieving of the soil suspension in water was done, and in the third stage of inoculation, mycorrhizal fungi inoculated to plants by Sorghum pot culture method. In the fourth stage, mixing of Verticillium with mycorrhizal fungi inoculum with soil was performed of olive seedlings tralanting was done. The effect of mycorrhizal fungi on olive Verticillium wilt disease traits (leaf number, branch number, plant height, fresh weight and dry olive branches and seedlings) in the statistical factorial design with four treatments in a greenhouse (sterilized soil only (control), sterilized soil+mycorrhiza, mycorrhiza+sterilized soil and Verticillium , Verticillium +sterilized soil) on olive Roghani and Zard varieties were studied. The results showed that mycorrhizal fungi in the presence of Verticillium , increased fresh weight and dry weight of olive branches and seedlings. It also increases the number of leaves and branches of olive seedlings compared to control treatment and Verticillium treatment in the absence of mycorrhiza. Statistical calculations and analysis of variance showed a significant differences between treatments observed (0.05 P) and were grouped based on LSD test was performed. Mycorrhizal fungi alone and in the absence of Verticillium in soil revealed a positive effect on traits in comparison to control. It seems that mycorrhizal fungi could be used as an appropriate solution to reduce the degree of olive Verticillium wilt disease.