Concrete is one of the most important construction materials due to availability, high compressive strength and pasty state before setting. However, having high compressive strength is not enough for durability; low tensile strength of concrete will cause cracks on its surface. Water and aggressive gases penetrate through cracks into the concrete and lead to corrosion of the reinforcement. To protect the concrete against cracks, chemical methods (epoxy or cement sand mortar) as well as biotechnological methods (calcium carbonate sediment produced by the bacteria) are used. In addition to being compatible with concrete, biotechnological methods are proved to be eco-friendly. Furthermore, adding bacteria to the concrete during the construction, repairs the cracks in the depth and at early ages. The researchers have concluded that concrete and cement hydration is harmful to bacteria, so bacteria must be protected against dry environment and alkaline concrete. One way of protection, is to add air bubbles in the concrete. Bacteria is shielded in air bubbles, where it can continue its activities. The air bubbles also provide oxygen for feeding bacteria. Therefore, different percentages of air (5%; 8%; and without intentional production of air) is used in this study. Using six different mixing methods, half of them containing Sporoscarcina pasteurii bacteria, we investigate compressive strength and electrical resistance (108 standard cylindrical specimens), water absorption (36 cubic specimens with dimensions of 70 mm), steel corrosion (36 cubic specimens of 70mm with rebar), the depth of carbonation (36 cubic specimens of 100mm), survival in cycles of freezing and thawing (36 cubic specimens with width and height of 100mm and length of 350mm), impermeability (18 cubic specimens with length and width of 200mm and height of 120mm) and accelerated chloride penetration (36 standard cylindrical specimens) of concrete. We set concrete with three different percentages of air (5%; 8%; and without intentional production of air) using Sporoscarcina pasteurii bacteria the construction of half of them. After the curing period, we performed surface restoration on some of the specimens, randomly selected. The results showed that calcium carbonate sentiment produced by bacteria fills the pores in concrete, reduces water absorption, corrosion of rebar and depth of carbonation. Furthermore, it reduces weight changes in successive cycles of freezing and thawing. Produced sediments restricted the free movement of ions in the pores of the concrete, reduced chloride ion penetration and increased the electrical resistance. Also, calcium-rich environment (curing environment containing minerals) improves bacteria’s performance. This results in increased compressive strength and electrical resistance and reduced water absorption, carbonation depth and permeability to water under pressure. Surface restoration generates a thin layer of calcium carbonate on the surface of concrete and limits the permeability, resulting in reduced penetration of chloride ions into the concrete interior which results in an increase in its electrical resistance. Presence of air bubbles in addition to bacteria decreases water absorption, carbonation depth and chloride penetration and dramatically increases electrical resistance. In fact, bacterial cells shielded by air bubbles from alkaline concrete and dry environment show better performance. Key words Air- entrained concrete, Bacteria, curing condition, Surface treatment, Time