Heavy metal pollution especially in soil is one of the problems of the environment. The increase in the concentration of heavy metals in soil is often due to human activities, Such as minery, industrial activities and the use of sewage. The complex of Irankouh mines is located in the South-West of Isfahan City. Considering these mines, the Surrounding areas are affected by the pollution caused by minery activities and environmental problems. Phytoremediation is one of the novel and potential methods of refining heavy metal polluted soils surrounding the miners and agricultural fields. In addition to being cost effective, natural and environmental friendly this technology is very suitable for the living organisms in soil and increases the biodiversity and is cable of maintaining the soil fertility. Promoting the plant and useful rhizosphere microorganisms relations which can increase the production of biomass and plants tolerance to heavy metals, are considered the main and most important methods to increase the efficiency of phytoremediation. In this greenhouse project, the effect of Eucslyptus species ( Eucslyptus camaladulensi ) and Arthrobacter agilis on the absorbtion of heavy metals such as Lead, Zinc and Cadmium and also the effect of Arthrobacter agilis on the s sorbtion ofthis heavy metal in the soil of “Bama” mine and the percentage of the absorbtion of them in different concentrations were studied. The effect of these heavy metals on the growth of Arthrobacter agilis in the media solution in different concentrations with the passing of time was also studied. The results showed that Arthrobacter agilis is able to absorb most of the heavy metals, Cadmium, Lead and Zinc compared to the control treatment (without any bacteria). The ability of these bacteria in absorbing lead is higher compared to Zinc and Cadmium. The percentage of cadmium absorbtion by Arthrobacter agilis in different concentrations (Cadmium 1, 2/5, 5 , 10 mg/l, Lead and Zinc 5, 50, 100, 200 mg/l) had no signification different and varied between 14 to 16 percent, while the amount of absorb Cadmium showed some increase with increase of the metal in the environment. The percentage of Lead absorption was decreased with the increase of this metal in the environment in high concentrations (200 mg/l). The same results were observed for Zinc in concentrations of 100 and 200 mg/l. The growth of Arthrobacter agilis was increased in the growth media during the time. The presence of Cadmium, Lead and Zinc in the environment caused a decrease in bacterial growth compared to the control treatment (without Cadmium, Lead and Zinc). The comparison between the Strilled treatments showed that the amount of biomass in the inoculated treatment was higher which states the positive effect of Arthrobacter agilis . The Stoma interchange in the inoculated treatments was higher with caused an increase in the amount of chlorophil, photosynthesis and growth. The amount of accumulation of heavy metals in root was higher compand to the shoot and the normal treatments showed higher accumulation compared to the Strilled treatments, which indicates the higher impact of local bacteria in soil compared to Arthrobacter agilis . The inoculated treatment showed higher heavy metal accumulation among the Strilled treatments that indicates the positive effect of Arthrobacter agilis . We can conclude that the presence of Arthrobacter agilis without the competition with other bacteria in soil can Lead to an increase in the heavy metal absorbtion in Eucalyptus with is impotent in the phytoremediation process. Keywords : Arthrobacter agilis , Eucalyptus camaldulensis , Phytoremediation, heavy metals, Bama mining