Today the world is facing energy crisis and limited resources of fossil fuel are unable to meet the growing demand for energy. Excessive use of fossil resources and the release of various pollutants have caused many environmental problems. This Issue along with rising fossil fuel prices and environmental pollution has prompted researchers to look for renewable and environmentally safe sources of energy. In this study, the biorefinery of Chlorella sorokiniana and its production of lutein were investigated. Chlorella sorokiniana microalgae were cultivated under photoautotrophic and photoheterotrophic modes and the effect of various parameters such as glucose concentration, nitrate concentration, light intensity and oxidative stress on lutein production were investigated. The photoautotrophic culture of this microalga was investigated in different percentages of normal air carbon dioxide and 3% carbon dioxide and different light intensities of 42 and 74 ?mol photons/m2.s. As a result, in photoautotrophic culture with light intensity of 47 ?mol photons/m2.s and 3% carbon dioxide, the highest amount of biomass equal to 1.38 g/L with 10.5% lipid content was produced. In photoheterotrophic culture with 2 g/L of glucose and exposure time of 12:12 light:dark with light intensity of 60 ?mol photons /m2.s, 1 g/L of biomass with 6.5% lipid content was produced. Increasing the light intensity to 200 ?mol photons/m2.s had the greatest effect on the lutein content. As a result, 11-day photoautotrophic single-stage culture at light intensity of 200 ?mol photons/m2.s with the production of 0.82 g/L biomass, 15.2% lipid and 1.45 mg lutein/gDW and two-stage culture by applying 4 days of light shock of 200 ?mol photons/m2.s with the production of 0.95 g/L biomass, 8.6% lipid and 3.29 mg lutein/gDW were selected as optimal values. Therefore, lutein content in single-stage and two-stage cultures was enhanced 6-fold and 10-fold, respectively, compared to normal culture without shock. In the next step, lutein and lipid were extracted simultaneously, as a result of which, 17% lipid and 1.17 mg lutein/gDW were obtained from single-stage culture and 12.65% lipid and 1.83 mg lutein/gDW were obtained from two-stage culture. Lutein content was decreased simultaneously in single-stage and two-stage cultures by 19.31% and 44.38%, respectively, and their lipid content was increased by 11.84% and 47.09%, respectively. Then lipid and lutein were extracted and bioethanol was produced from the remaining biomass. The highest amount of ethanol from remaining solids of simultaneous extraction of lutein and lipids in single-stage culture was obtained 6.13 g/L ethanol with a theoretical yield of 23.33% or 0.123 g ethanol/g solid residue after 48 h fermentation with Saccharomyces cerevisiae yeast. While from the raw biomass of microalgae from single-stage culture, 3.51 g/L ethanol or 0.07 g ethanol/gDW was obtained after 48 h fermentation. Finally, from 1 g dry microalgae biomass obtained from two-stage light shock with simultaneous extraction of both products, 0.002 g lutein and 0.13 g lipid and from the 0.87 g of the remaining solid, 0.12 g ethanol were produced.