Spacer structures are knitted fabric constructions comprising two separate fabric webs which are joined together by spacer threads. The spacer threads are generally made of PET or PA monofilament yarns. These fabrics because of their special properties have many applications such as protective and sports clothing and shoes, seat covers, bandages. In order to investigate the dynamic moisture transfer of spacer fabrics, an experimental apparatus has been developed which enable the simulation sweating of human body. It consists of a controlled environmental chamber, guarded hot plate, and humidity sensors and data acquisition system. With comparison of moisture transfer between spacer fabrics and leather, it is obvious that moisture transfer of spacer fabric is very higher than moisture transfer of leather. Then, with comparison of different samples, the influence of some parameters such as heat setting, the kind of spacer threads, air permeability, thickness and porosity on moisture traort properties are investigated. Spacer fabrics well able to traort the moisture increase in the microclimate close to the skin during intermittent perspiration of the human subject, and they also guarantee excellent wear comfort. Heat setting under drawing of spacer fabrics increases the porosity and air permeability and decreases thickness and weight per unit area. With Increasing of porosity and air permeability, moisture traort is increased. The Results shows that water vapor produced by sweating can be easily and quickly transferred from next to the skin to the outer surface to keep the skin dry. A mathematical simulation of moisture diffusion into spacer fabrics is developed and the model is solved numerically by the finite difference method. The results of water vapor concentration in air and water vapor concentration in fibers are illustrated by a series of 3D diagrams in terms of time and different thickness of fabric.