Cellulose nanofibers film, have recently attracted considerable attention owing to their outstanding strength, high traarency, and good barrier properties, resulting in a wide range of applications such as biocomposites and packaging. In this work, Cellulose nanofibers film with the thickness of 10-14 microns were prepared from cellulose nanofibers suspension and glycerol as the plasticizer by solvent casting and vacuum filtration methods. The evaluation of the light traarency showed that the addition of glycerol up to 0.1 wt.% slightly affects the traarency of the Cellulose nanofibers film. Also, the water vapor permeability test has revealed that the presence of glycerol increases the water absorption of the film. The X-ray diffraction (XRD) patterns showed that the preparation methods have no considerable effect on the Cellulose nanofibers film crystallinity. However, the results of the atomic force microscopy (AFM) analysis revealed that the Cellulose nanofibers film prepared by vacuum filtration method have a rougher surface than the films prepared by the other method, which further confirmed by static contact angle studies. The mechanical properties of the Cellulose nanofibers film were evaluated using tensile test. The results indicated that the Cellulose nanofibers film have the tensile strength and modulus of 86.65 MPa and 10.6 GPa, respectively. The vacuum filtrated Cellulose nanofibers film were used as the reinforcement layers to prepare the epoxy laminated composites, and the mechanical properties of the single-layer and bi-layer composites were characterized and compared. The results showed that the addition of two Cellulose nanofibers film layer significantly improved the mechanical properties of the matrix and the strength and elongation were increased about 213% and 134.5% in comparison with the single layer composite. Furthermore, the Cellulose nanofibers film addition increased the impact resistance of the epoxy matrix by 58.88%. Then, the clay nanoparticles, closite 30B, were added to the matrix (0.5-3 wt.%) and the properties of two- Cellulose nanofibers film - layer laminated composites were characterized by field-emission scanning electron microscopy (FE-SEM), and thermogravimetric analysis(TGA). The XRD patterns were used to study the distribution of clay nanoparticles in epoxy resin .Also, in order to investigate the effect of clay nanoparticles on the mechanical properties of the laminated composites, the impact strength and tensile tests were conducted. The results showed that the incorporation of 2wt.% closite 30B have a significant effect on the mechanical properties of the laminated composites and the tensile and flexural strength and modulus and impact strength of the samples increased by 116.5 MPa,87.2 MPa,4.8 GPa,5.2 GPa and 243.3 kJ/m2 ,respectively.