This study was conducted to identify quantitative trait loci (QTLs) controlling important agronomic traits and map marker enrichment in 99 F 13 recombinant inbred lines (RILs) derived from barley cross Azumamugi × Kanto Nakate Gold. The parents and inbred lines were evaluated for agronomic characteristics including days to heading, plant height, peduncle length, length and width of flag leaf, spike number per area, kernel number per spike, kernel weight per spike, spike weight, spike length, awn length, biomass, kernel yield, harvest index, 1000-kernel weight and test weight. Field trails were conducted as randomized complete block design with two and three replications in 2003 and 2004, respectively. Significant differences and transgrassive segregants were observed among lines for all of the traits. To identify QTLs, composite interval mapping (CIM) was done based on linkage map constructed using 99 RILs and 2 morphological, 2 isozyme, 34 STS and 62 AFLP markers. New QTLs controlling days to heading, plant height, peduncle length, spike number per m 2 , spike length, biomass, kernel yield, test weight and harvest index were identified in this population. A strong QTL controlling 25% of phenotypic variation of days to heading was detected in the map interval of e07m25.3-e12m199.1 markers on chromosome 5HL. A strong QTL for width of flag leaf was detected in the map interval of ABA001-uzu on chromosome 3HL which explained 20% of the phenotypic variation. A strong QTL for spike number per m 2 , explaining 10% of the phenotypic variation, was located in the map interval of ABG604-e09m28.8.2 on chromosome 5HL. Most of the phenotypic variation for plant height, peduncle length, spike length and awn length was explained by the QTLs detected in the map interval of uzu-e06m30.10.1 on chromosome 3HL.The QTLs identified in the map interval of ABA001-uzu and uzu-e06m30.10.1 markers on chromosome 3H could explain 12% of the biomass phenotypic variation. The QTLs detected in the map interval of vrs1-MWG503 on chromosome 2HL could explain most of the phenotypic variation for kernel number per spike, spike weight, kernel yield, harvest index and 1000-kernel weight. Most of the phenotypic variation for kernel weight per spike was explained by the QTLs located in the map interval of vrs1-MWG503 and MWG801-vrs1 on chromosome 2H. The results revealed that important QTLs controlling most of the traits were located near by the uzu1 and vrs1 loci. Also high correspondence was found for the QTLs located on chromosomes 1HL, 2HS, 2HL, 1HL, 3HS, 4HS, 4HL, 5HL, 4HL, 4HS and 7HS with eam
