This study was conducted to saturate the linkage map of wheat using the AFLP markers and also mapping the QTLs for some important quantitative traits such as, plant height, heading date, date to maturity, grain yield and yield components. A doubled haploid population derived from crossing of two cultivars, Fukuho-kumogi and Oligo-culm was used. The parental lines and 157 lines were evaluated in a randomized complete block design with three replications at the research farm of Isfahan University of Technology in two years of 1382 and 1383. The analysis of variance in each year and combining the data over years for the agronomic and morphological traits showed significant differences among the genotypes for all of the traits. The estimation of genetic and phenotypic coefficients of variations indicated that there was more genetic variation for plant height, peduncle length, number of grains and grain weight per spike, spikes per m 2 , spike length and grain yield. The estimation of heritability for plant height, heading date, number of grains per spike, grain weight per spike and date to maturity were 98.0%, 88.7%, 87.3%, 85.9% and 80.3% respectively. The AFLP analysis using 11 primer combinations of Pst I+ANN- Mse I+CNN amplified 66 polymorphic bands between the parents, but 48 markers were assigned to the wheat linkage groups. The mean of amplified polymorphic bands was 6 per each primer combination. The primer combinations of Pst I+AAT- Mse I+CCC, Pst I+AAT- Mse I+CAC and Pst I+AGA- Mse I+CCC each with 9 bands had higher polymorphic bands. In this study, 41% of AFLP markers assigned to the linkage groups of genome A, 43% to genome B and 12% to genome D. The results of mapping QTLs for the studied traits indicated the expression of the permanent QTLs with main effects for plant height, peduncle length, spike length, spikes per m 2 , number of grains per spike, grain weight per spike and heading date. The results showed two QTLs with main effects were located in the expected location of Rht1 and Rht2 genes on the chromosomes 4B and 4D for plant height that explained 60.3% and 61.1% of phenotypic variation of this trait in 1382 and 1383, respectively. In the interval of 29.0 to 34.0 centimorgan from the beginning of chromosome 2D and close to the Xcfd53 and Xgwm261 markers, the QTLs for awn length (R 2 =11.0%), spike length (R 2 =31.0%), plant height (R 2 =10.8%), peduncle length (three QTLs with R 2 = 7.5%), date to maturity (R 2 =11.0%), pollination date (R 2 =10.7%), harvest index (R 2 =12.4%), grain yield (two QTLs with R 2 =6.5%), spikelete compactness (R 2 =18%), number of grains per spike (R 2 =3.5%) and grain