Iran with various climatic conditions is one of the most important countries in the cultivation of fruit trees especially stone fruits in the world. Many viruses have been reported on stone fruits around the world so far. Plum pox virus (PPV) and Tomato ringspot virus (ToRSV) are two major viruses in stone fruits. PPV is a member of the Potyvirus genus and Potyviridae family with a positive-sense single-stranded RNA genome. The virus causes Sharka disease, one of the most destructive diseases of stone fruits, worldwide. PPV is transmitted by aphid in a non-persistent manner and vegetative organs. Tomato ringspot virus (ToRSV) is a member of the Nepovirus genus and Secoviridae family with isometric particles and two positive-sense single-stranded RNAs as genome. The virus has wide host range mainly on woody and ornamental plants. ToRSV is transmitted by nematode, seeds, pollen and vegetative organs. There is no information on the presence of PPV and ToRSV on stone fruits in Isfahan province. In this research to detect PPV and ToRSV a survey was carried out during 2017-2019. Symptomless leaf samples and the leaf samples showing viral symptoms were collected from stone fruits orchards in Isfahan province. Total RNA was extracted from leaf samples and tested by RT-PCR using specific primer pairs for PPV (P3D/P4B and P1/P2) and ToRSV (ToRSV-D1/ToRSV-U1 and Oligo1n/Oligo2n). Some collected samples were tested using double-antibody sandwich enzyme-linked immunosorbent assay (DAS-ELISA) using a commercial polyclonal antibody against PPV. In RT-PCR unexpected fragment sizes of 400 bp and 500 bp were amplified in one and two of the 169 samples using ToRSV-D1/ToRSV-U1 and Oligo1n/Oligo2n primer pairs, respectively. The amplicons were sequenced. No homology was found in BLAST search between sequenced samples and ToRSV isolates available at GenBank. In DAS-ELISA eight of the 55 samples collected from Khansar were tested positive. The expected PCR product of 243 bp was amplified in five of the 313 samples in RT-PCR using P1/P2 primer pair. No expected PCR product was amplified in tested samples using P3D/P4B primer pair. The amplicons were weak and despite changing the time and temperature of annealing phase and MgCl 2 concentration in PCR, no change was found in intensity of amplified bands. The PCR products were extracted from gel and sequenced. Analysis of sequenced samples by Chromas ver. 2.6.4 showed low-quality of sequences so their BLAST search results are unreliable. In this study ToRSV was not detected on stone fruits in Isfahan province, but based on ELISA and RT-PCR results PPV probably exist in Isfahan province on stone fruits, which is required to confirm by sequencing of amplified bands. Due to the serological relationship between potyviruses, the probability of presence of a virus close to PPV should be considered in Isfahan province. Keywords: Stone fruits, Plum pox virus, Tomato ring spot virus,ELISA, RT-PCR