This work was aimed to investigate the potential preparation of an active biodegradable film from bitter vetch seed proteins and determine some of its physicochemical properties. The film was cast from bitter vetch protein concentrate (BPC) (5 g/ 100 ml alkaline water) and glycerol (50% w/w of BPC). The effects of CaCl 2 , oxidized ferulic acid (OFA) and pomegranate juice on the BPC-based film properties were also examined. Finally, the effect of BPC-based film especially films containing pomegranate juice was evaluated on oxidation of sunflower oil. According to the results, the BPC-based film with moisture content (MC) of 27.69%, total soluble matter (TSM) 36.57%, tensile strength (TS) 5.04 MPa, elongation at break (EB) 118% , water vapour permeability 0.72 g.mm/m 2 hkpa, surface hydrophobicity 49.83 ? , surface roughness 10.63 nm and antioxidant activity 22.56% had physicochemical properties comparable to or even better than other biodegradable films. CaCl 2 and OFA improved barrier and mechanical properties of the films while the pomegranate juice-containing films showed increased antioxidant activity compared to the control samples. Also the BPC-based film due to the relatively good antioxidant activity had a positive effect on reducing the oxidation of sunflower oil and if some modifications would be done, particularly the water vapor permeability, it could be suitable for use in food packaging. Key Words Bitter vetch; Biodegradable film; Pomegranate juice; Barrier properties; Sunflower oil.
