Synthetic polymers as petroleum-based products are widely used in a variety of packaging materials; however, due to air pollution and a great decrease in fossil fuels, a huge number of researches have been done to see if synthetic plastics can be replaced by those obtained from renewable energy sources. Among those sources, biopolymers are the most attractive ones owing to both their availability and biodegradability. An outstanding example of this group is corn zein which is highly potential for such an aim and numerous studies in the area of packaging are allocated to this material. In spite of the above merits, biopolymers have some demerits which limit their application as an alternative to synthetic polymers. These disadvantages include low mechanical strength, high gas permeability especially to water vapor, low thermal resistance and being sensitive to long processing operations. A way to dominate the biopolymers’ weaknesses is to employ fillers with particular properties. Today, biopolymers reinforced with nanoclays (nanocomposites) are considered as a good alternative to traditional composites and they show higher mechanical and physical properties in comparison with biopolymers without fillers or micro composites. Bearing in mind that during direct contact between food products and packaging materials, some types of substances might migrate to the food product, it is necessary to check all the new packaging materials to see if the materials are appropriate for being directly or indirectly in touch with the food. So, in this study a nanocomposite film composed of zein reinforced with modified montmorillonite is produced and subsequently its mechanical, thermal and barrier properties are investigated. The rate of Silicon migration (as a montmorillonite indicator) from nanocomposite films (0, 3 and 5 %) to food stimulants (water and acetic acid 3%) in 40 o C and during 30 days of storage is also measured using Graphite Furnace Atomic Absorption Spectrometry (GFAAS). Based on X-ray diffraction results, the film structure displays exfoliation. The data reveal that adding a little amount of montmorillonite, the zein film’s mechanical, thermal and barrier properties increase. Migration test proves that the value of Silicon migration to aquatic environment in the zein film excluding montmorillonite change from 0.24 to 0.22 mg/lit during the 10 th day to 30 th day of storage; while, this increases from 0.3 to 0.47 and from 0.32 to 0.47 in the case of zein reinforced with 3 and 5% filler, respectively. During the 10 th day to 30 th day of storage, the value of migration to acidic environment has changed from 0.37 to 0.32, 0.37 to 0.62 and 0.48 to 0.97 in the case of zein without filler, zein reinforced with 3 and 5% filler, respectively. As a result, the type of food stimulant, the amount of reinforcement and the time of storage period affect the value of migration significantly. Based on the obtained data, the rate of migration to acidic food stimulant is more than what happens in aquatic one which can be attributed to the higher solubility of the film and Silicon in acid. Moreover, as the amount of filler increases, migration grows which is completely expectable. Additionally, the longer the storage lasts, the more the migration happens. These findings would provide beneficial information related to employing nanocomposite of zein and montmorillonite as a packaging materials. Keywords: zein, nanocomposite films, montmorillonite, migration