"Water is the source of life" is the best phrase about water importance. Today, with the industrialization of human societies, dangerous pollutants, including synthetic dyes, enter industrial effluents. Therefore, providing effluent treatment methods to remove maximum contaminants is very important. Photocatalysis is an eco-friendly method that is a remarkable aptitude for the degradation of many organic pollutants. In this method, photocatalysts decompose organic pollutants into harmless substances under light radiation through advanced oxidation reactions. In this research, Polyacrylonitrile (PAN) nanofibers containing layered double hydroxide (LDH) have been used for photocatalytic degradation of methyl orange and reactive blue 13 dye. For this purpose, the first LDH was syntheses using the co-precipitation method. Then PAN polymer solutions with a concentration of 9, 11, and 13 % wt. containing different amounts of LDH (0, 2, 4, 6, and 8 % wt.) were electrospun. The FTIR and XRD results showed the successful formation of LDH. Besides, EDX results confirmed the existence of LDH nanolayers in the nanofibers. Based on the FESEM images, the nanofibers' diameter was decreased by reducing the polymer solution's concentration. Also, by adding 2% LDH to the polymer solution, the nanofibers' diameter increased, but increasing the concentration of LDH from 2 to 4, 6, and 8% reduced the nanofibers' diameter. According to the results, it was observed that nanofibers obtained from 13% wt. PAN solution containing 4% wt. LDH gave the highest photocatalytic degradation efficiency of methyl orange. The TGA results showed that the thermal stability of PAN-LDH nanofibers increased compared to pure PAN nanofibers. The PL results show a decrease in UV and visible emissions due to loading LDH on PAN nanofibers, which hinders the recombination of photoexcited electron and hole and thus improves the photocatalytic activity of PAN-LDH nanofibers. The photocatalytic degradation activity of reactive blue dye 13 increased with increasing LDH concentration and weight of nanofibers. The best results were obtained with 100 mg nanofibers containing 4% LDH. The effect of reactive blue 13 dye solution pH on the photocatalytic activity of nanofibers was also investigated. The highest efficiency of degradation of reactive blue 13 dye was obtained in an acidic environment (pH=4). The equilibrium data were more consistent with the pseudo-first-order kinetic model concerning the reactive blue 13 dye degradation by PAN-LDH photocatalytic nanofibers. The Freundlich isotherm was also a more conclusive model for describing experimental data. The results of COD measurements of effluents after photocatalytic degradation of reactive blue 13 dye showed that the quality of the resulting effluent in addition to decolorization has improved. Dye photocatalytic degradation of PAN-LDH nanofibers remained almost constant four times for reuse.Finally, the photocatalytic properties of PAN nanofibers containing LDH, were investigated for photocatalytic degradation of the actual dyeing effluent sample. In addition, the effluent COD was measured before and after the photocatalytic proce