The present study aimed to improve the efficiency of microspore culture for production of doubled haploid lines (DHs) and to evaluate the resultant DH lines for salinity tolerance under in vitro and greenhouse conditions. Four genotypes including, H 1 and H 2 hybrids and RGs003 and Okapi cultivars were used. Results showed that H 1 and were more responsive to microspore culture and addition of 13% sucrose to the NLN induction medium performed better than other concentrations of 10 and 17% for embryogenesis. Adding 0.1 mgl -1 GA3 in to B 2 medium caused a positive effect on plant regeneration. Results of in vitro salinity assessment revealed the significant variation among DH lines for salinity tolerance at cellular level, with DH lines 13, 78, 25, 68, 16 and 21 identified as being salt-tolerant. Cluster analysis divided 16, 13, 21, 25, 68, 58 and 36 lines in to a single group possessing the highest dry weight, fresh weight, plant height, K + and Ca 2+ concentration, K + /Na + ratio and Ca 2+ /Na + ratio, and regarded as being salt-tolerant at greenhouse conditions. In conclusion, our data suggest that DH lines 13 and 16 were the most salt tolerant and DH lines 24 and 97 were the most salt sensitive. Further studies are needed to better elucidate the physiological mechanism and genetic basis underlying salinity tolerance in these lines, and ultimately their utilization in canola breeding program can be recommended. Key words : canola, microspore culture, doubled haploid, salinity, In Vitro culture
