Environmental contamination attributed to the presence of organic and inorganic pollutants is one of the major problems in the world. Benzene is an organic chemical compound with the molecular formula C6H6. It is a natural constituent of crude oil, and is one of the most elementary petrochemicals. Benzene can enter the human organism through inhalation, ingestion or skin contact and may accumulate in bones, brain, kidney causing serious damage to the kidney, liver and nervous system. It causes anemia, cancer and sometimes even death. Due to the dangerous effects of benzene it is essential to check waste streams containing benzene before being discharged into the water resources. The maximum permissible limit assigned by World Health Organization (WHO) for benzene in drinking water is 0.001 mg/L (WHO, 1996). In recent years, attempts are therefore being made to develop inexpensive adsorbents utilizing abundant natural materials. Agricultural waste materials often employed as adsorbent may have potential marketing preference for wastewater treatment among other adsorbent types due to the low cost, environmentally friendly, naturally accessible and efficient. Using zero-valent iron nanoparticles (nZVI) has attracted attentions in the field of environmental pollution. For this purpose, using nZVI as one of the recent new technologies can be useful. These particles are very reactive as their sizes are very small. nZVI effectively reduces organic and inorganic pollutants, but their mobility and lifetime are limited. In this study, the ash cone pine(APC) and ash modified with nano zero-valent iron (APC+nZVI) were used for removal of benzene from aqueous solutions. Techniques such as XRD, FTIR, SEM and EDX were used to characterize the adsorbent attribute of the APC and APC+nZVI and its ability as an adsorbent, while variable initial concentration of benzene, the amount of adsorbent, contact time, temperature and pollutant's solution pH were investigated. Isotherm models, Langmuir and Freundlich were fitted to benzene adsorption equiblirium data. Kinetic models, pseudo first order, pseudo second order, intra particle diffusion and power function were used to describe kinetic data of benzene adsorption. Optimum benzene adsorption was observed at pH: 7 and optimum amount of adsorbent 0.1 g for APC and APC+nZVI. The observed equilibrium time on APC and APC+nZVI was 10 min and 300 min, respectively while, the equilibrium adsorption capacities were 366 mg/g and 392 mg/g at 2000 mg/L initial benzene concentration. Linear and non-linear isotherm studies showed that equilibrium data better fitted for APC to Langmuir and for APC+nZVI to Freundlich isotherm model. Kinetic studies showed better applicability of pseudo-second order kinetics model. It was concluded that APC+nZVI showed better performance for benzene removal compared to APC. To complete the studies on the introduced adsorbent, column adsorption experiments were performed to check the absorbent performance during continuously injected benzene solution into the adsorbent column, until the adsorbent has been saturated. The results for columns with continuous inflow indicated that the maximum capacity of adsorption of benzene, for adsorbent column with diameter of 3cm and input concentration of 1000 mg/L and input rate of 100 mL/h, for APC and APC+nZVI was 295 mg/g and 392 mg/g. The results of this experiment