The tomato leafminer, Tuta absoluta (Lepidoptera: Gelechiidae), is a major pest in tomato in greenhouses and fields in Iran. The use of sex pheromone of T. absoluta , together with releases of the parasitoids wa of the Trichogramma genus (Hym .: Trichogrammatidae) are here considered as a control strategy against T. absoluta. Howevere, because the behavior of several species of parasitoids is known to be affected by the sex pheromones of their hosts, the possible indirect effects of pheromone-based control of the pest insect on their natural enemies should also be considered when using pheromones for pest control. In this work, responses of female wa of three populations of T. evanescens , Wolbachia uninfected (W?) T. brassicae and Wolbachia infected (W?) T. brassicae to sex pheromone of T. absoluta were studied under laboratory conditions. The results revealed that only female wa of T. evanescens and T. brassicae W? were attracted to synthetic pheromone and calling virgin female moths around 62% and 75%, respectively. Also their responses were affected by the wasp age and ambient temperature. One-days old wa were attracted more than three and five-day old wa to the sex pheromone and their responses at 30 °C were more than that of 35 °C and 25°C. T. brassicae W ? did not respond to the sex pheromone of the T. absoluta . Investigating the effect of experience (previous exposure to the host pheromone) showed that in T. evanescence and T. brassicae W? experience reduced the response to sex pheromone but in T. brassicae W ? enhanced it. The fertility life table parameters were assessed for three populations under two different treatments, synthetic pheromone release and control. The results showed that several life table parameters were reduced under the pheromone treatment, for example in T. brassicae W? net reproductive rate (R0), the gross reproductive rate (Grr), generation time (T), age-specific survival rate (lx), female longevity, percentage of adult emergence and the number of parasitized eggs per female, in T. brassicae W ? age-specific survival rate (lx), female longevity and percentage of adult emergence and in T. evanescence age-specific survival rate (lx), but significant changes were not observed in the other parameters. Attraction of parasitoids to the synthetic sex pheromone of T. absoluta was measured in the greenhouse conditions. There was no record of the parasitoid in pheromone traps. These results help to determine integrated pest management program for control of T. absoluta . Key words: Egg parasitoids, Sex pheromone, Olfactometer, Fertility life table, Mating behavior
