The number of three experiments was conducted to evaluate some herbicide treatments and drought tolerance of two Salvia species (Salvia officinalis and Salvia sahendica) and also responses of these two species to ascorbic acid and putrescine as booster of defense system. In the first experiment, the number of five photosynthetic inhibitor herbicides (oxadiargyl, bentazon, oxyfluorfen, metribuzin, phenmedipham +desmedipham) with three rates (0.75X, 1X and 1.25X that X equals to the recommended rate) on two Salvia ecies was conducted as a two-way factorial arranged in a randomized complete block design with three replications. In the field experiment, effect of ascorbic acid and putrescine (0 and 200 mg/L) on two mentioned Salvia species under three levels of irrigation (control, moderate and severe drought: respectively 50%, 70%, 85% depletion of available soil moisture) was conducted as a split-plot randomized complete block with 3-replicate. The third pot experiment was a 4-replicates factorial completely randomized design, including S. officinalis, the two irrigation levels (control and moderate stress), ascorbic acid (0 and 200 mg/L) and three rate of bentazon (control, 1X and 1.25X that X equals to the recommended rate). Based on the morphophysiological traits were measured, herbicidal treatments discriminated into three groups, first group: low and recommended rate of phenmedipham +desmedipham, bentazon, oxyfluorfen, oxadiargyl that two mentioned Salvia ecies were tolerant, second group: high rate of phenmedipham +desmedipham, bentazon, oxyfluorfen, oxadiargyl that two mentioned Salvia ecies were moderately tolerant and third group: all rate of metribuzin that two mentioned Salvia ecies were sensitive. In two species antioxidant enzyme activity increased with herbicide rate increased until recommended rate then decreased, but on the other hand, the antioxidant enzyme activity may be neutralized by the toxic effects of the herbicides, with the exception of metribuzin treatments. Drought stress led to increased hydrogen peroxide, malondealdehyde, enzayme activity, phenolic compounds, proline and essential oil concentrations but decrease photosynthetic pigments concentration, relative water content, height and biological yield in both species. Drought stress led to increase in irrigation water use efficiency (IWUE) for biological yield and essential oil yield in moderate and severe stress in S. officinalis but there was no differences between irrigation levels in IWUE for S. sahendica biological yield. The biological yield of S. officinalis and S. sahendica decreased from 10-22% and 16-32% respectivley. Foliar-applied ascorbic acid led to increase in biological yield of S. officinalis and S. sahendica 17% and 4.5% respectively and putrescine led to increase in biological yield of S. officinalis and S. sahendica 4% and 4.5% respectively. Finally it can be concluded that drought induced harmful effects on Salvia but it could be alleviated by ascorbic acid and putrescine. In third experiment, simultaneous application of drought and herbicide stress led to morphophysiological alterations on S. officinalis, so that led to decrease shoot and root dry weight, essential oil yield and irrigation water use efficiency for shoot dry weight 33%, 31%, 18 and 21% respectively. Although ascorbic acid did not notable effects on relative water content, malondealdehyde, photosynthetic pigments concentration and catalas activity but increased prolin, hydrogen peroxide, ascorbate peroxidase, peroxidase, height, shoot and root dry weight, essential oil concentration and yield 72%, 23%, 14%, 63%, 7%, 28%, 30%, 20% and 41% respectively. With increasing bentazon rates, prolin, phenolic compound, hydrogen peroxide and malondealdehyde increased until recommended rate then decreased. phytotoxicity symptoms were stable until 30 days after treatment while the plant recovery was started. The herbicide phytotoxicity at recommended rate reduced by drought stress but at the high rate not only did not reduce by drought stress but also reduced shoot dry weight because of synchronize two intense tensions together. However ascorbic acid alleviated the stress impacts but synchronization of two kind of stress (drought and bentazon herbicide) led to plant recovery delayed. As a whole, it can be concluded that Salvia damaged by drought and herbicide application especially S. sahendica but the damage intensity depend on irrigation regime, herbicide type and rate. It seems that under low irrigation conditions, commercial production of the Salvia with pre-treatment by ascorbic acid and reduced bentazon rate is feasible. Key Words: Herbicide tolerance, oxidative stress, lamiaceae family, essential oil, irrigation water use efficiency. Key Words: Herbicide tolerance, oxidative stress, lamiaceae family, essential oil, irrigation water use efficiency.