Due to the impact of greenhouse gases such as carbon dioxide in the atmosphere as well as reuse of carbon dioxide for different purposes, gas separation method has great importance in the industry. In the present study to learn more about this phenomenon, separation of carbon dioxide gas by means of hydrate equilibrium conditions (temperature and pressure equilibrium) and the mixture of nitrogen and carbon dioxide gases with hydrate promoters were studied in the presence of acetone and ammonium tetrabutylammoniumchloride salt. In this method for experimentation a cell with jacket made of stainless steel with volume of 509 ml were used. Temperature and pressure were measured and reported by a sensor cell and electronic board connected to a computer. First, 200 ml of pure water was used in order to find hydrate equilibrium points for nitrogen and CO 2 (44.31 mol. %). The equilibrium pressure and temperature was 34 bar and 7 ?C and the percentage of separation was 41.53. Then, carbon dioxide and nitrogen gases at different initial pressures in the presence of acetone (initial temperature, stirrer speed, volume of the solution and quickly change the same temperature) were experimented. It was observed that the initial pressure reduced from 37 bar to bar 29 and the equilibrium temperature and pressure was changed from 8 ?C and 32 bar to 2.5 ?C and 24 bar so the separation was changed from 38.25 to 32.62 %. By reducing the pressure from bar 29 to bar 26, the temperature and the pressure equilibrium was changed from 24 bar and 2 ?C to 18 bar and 2.5 ?C so that the separation was reduced from 32.62 to 31.85 % which implies that the equilibrium temperature and pressure and percentage of carbon dioxide was declined. Acetone and tetrabutylammoniumchloride salt was used for gas hydrate formation with varying proportions of carbon dioxide in mixture of carbon dioxide and nitrogen. The best equilibrium conditions and separated carbon dioxide was 18.5 ?C, 5.26 bar and 53.54 % and it was observed that the mixture of acetone and tetrabutylammoniumchloride salt by weight of 24 and 5 % was used at the same condition (initial pressure and temperature, stirrer speed, volume of solution and the change of temperature). When the water was used in the absence of tetrabutylammoniumchloride and acetone at the same condition (initial pressure and temperature, stirrer speed, volume and rate of temperature change of the same solution), the lowest carbon dioxide separation equal to 41.53% and the worst equilibrium points that was the 7 ?C and 34 bar was observed. Consequently the results showed acetone and tetrabutylammoniumchloride improves equilibrium conditions and also helped to increase the separation of carbon dioxide indicating a healing effect of hydrate formation thermodynamically. Keyword: Carbon dioxide Hydrate, Gas Hydrate Promptor, TBAC, Acetone